| Patterns of Diabetic Periodontal Wound Repair: A Study Utilizing Micro-Computed Tomography and Immunohistochemistry. | |
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MedLine Citation:
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PMID: 21966943 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Background: Diabetes is known to impair wound healing and deteriorate the periodontal condition. There is limited information about the patterns and events associated with periodontal wound repair. In this study, we evaluate the dynamics of periodontal wound repair using micro-computed tomography (micro-CT) and immunohistochemistry. Methods: 36 male rats were utilized, and diabetes was induced by streptozotocin. The maxillary first molars were extracted, and a tooth-associated osseous defect was created in the extraction area. Animals were sacrificed after 7, 14, and 21 days. Volumetry and distribution of bone trabeculae were evaluated by micro-CT imaging. The patterns of healing and collagen alignment were evaluated by histology. Advanced glycation end-product (AGE) deposition and expression of the receptor for AGEs (RAGE), tartarte-resistant acid phosphatase (TRAP), and proliferating cell nuclear antigen (PCNA) were evaluated by histochemical and immunohistochemical staining. Results: Diabetic animals demonstrated a significantly reduced bone volume and trabecular number as well as thinner trabeculae and more trabecular separation in osseous defects. The early stage was characterized by significantly reduced cellular proliferation and prolonged active inflammation without evident bone resorption, whereas delayed recovery of collagen re-alignment, matrix deposition, and bone turnover was noted in later stages. Although AGEs and RAGE were present during healing in diabetes and controls, a stronger and more persistent level of expression was observed in the diabetic group. Conclusions: Diabetes significantly delayed osseous defect healing by augmenting inflammation, impairing proliferation, and delaying bone resorption. The AGE-RAGE axis can be activated under metabolic disturbance and inflammation. |
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Authors:
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Po-Chun Chang; Min-Chun Chung; Yi-Ping Wang; Li-Ying Chien; Jason C Lim; Kaicheng Liang; Li Yen Chong; Yen-Ping Kuo; Chun-Hao Chen; Huan-Ching Chiang |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-10-3 |
Journal Detail:
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Title: Journal of periodontology Volume: - ISSN: 1943-3670 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-10-4 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8000345 Medline TA: J Periodontol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Faculty of Dentistry, National University of Singapore, Singapore, Singapore. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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