Document Detail

Patterning human stem cells and endothelial cells with laser printing for cardiac regeneration.
MedLine Citation:
PMID:  21911255     Owner:  NLM     Status:  Publisher    
Recent study showed that mesenchymal stem cells (MSC) could inhibit apoptosis of endothelial cells in hypoxic condition, increase their survival, and stimulate the angiogenesis process. In this project we applied Laser-Induced-Forward-Transfer (LIFT) cell printing technique and prepared a cardiac patch seeded with human umbilical vein endothelial cells (HUVEC) and human MSC (hMSC) in a defined pattern for cardiac regeneration. We seeded HUVEC and hMSC in a defined pattern on a Polyester urethane urea (PEUU) cardiac patch. On control patches an equal amount of cells was randomly seeded without LIFT. Patches were cultivated in vitro or transplanted in vivo to the infarcted zone of rat hearts after LAD-ligation. Cardiac performance was measured by left ventricular catheterization 8 weeks post infarction. Thereafter hearts were perfused with fluorescein tomato lectin for the assessment of functional blood vessels and stored for histology analyses. We demonstrated that LIFT-derived cell seeding pattern definitely modified growth characteristics of co-cultured HUVEC and hMSC leading to increased vessel formation and found significant functional improvement of infarcted hearts following transplantation of a LIFT-tissue engineered cardiac patch. Further, we could show enhanced capillary density and integration of human cells into the functionally connected vessels of murine vascular system. LIFT-based Tissue Engineering of cardiac patches for the treatment of myocardial infarction might improve wound healing and functional preservation.
Ralf Gaebel; Nan Ma; Jun Liu; Jianjun Guan; Lothar Koch; Christian Klopsch; Martin Gruene; Anita Toelk; Weiwei Wang; Peter Mark; Feng Wang; Boris Chichkov; Wenzhong Li; Gustav Steinhoff
Related Documents :
11559675 - Ten year mortality in subsets of patients with an acute coronary syndrome.
8248465 - The influence of psychosocial factors on survival after myocardial infarction.
12935615 - Lung-heart weight ratio as a possible index of cardiopulmonary pathophysiology in drown...
17322625 - Long-term nitrate therapy after acute myocardial infarction does not improve or aggrava...
17069905 - High sensitive c-reactive protein and interleukin 6 are not related to neointimal hyper...
20521015 - Predictors of restenosis after percutaneous coronary intervention using bare-metal sten...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-9-10
Journal Detail:
Title:  Biomaterials     Volume:  -     ISSN:  1878-5905     ISO Abbreviation:  -     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-9-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier Ltd.
Reference- and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, 18057 Rostock, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The effects of aggregation and protein corona on the cellular internalization of iron oxide nanopart...
Next Document:  The effects of the physical properties of culture substrates on the growth and differentiation of hu...