Document Detail


Patterning chemical stimulation of reconstructed neuronal networks.
MedLine Citation:
PMID:  17723603     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
A spatially resolved delivery of substances integrated with cell culture substrates shows promise for application in pharmacological assays, bioanalytical studies on cell signaling pathways and cell-based biosensors, where control over the extracellular biochemical environment with a cellular resolution is desirable. In this work, we studied a biohybrid system where rat embryonic cortical neuronal networks are reconstructed on microstructured silicon chips and interfaced to microfluidics. The design of cell-cell and cell-medium interactions in confined geometries is presented. We developed an aligned microcontact printing technique (AmicroCP) for poly(lysine)-extracellular matrix proteins on microstructured chips, which allows a high degree of geometrical control over the network architecture and alignment of the neuronal network with the microfluidic features of a substrate. Spatially resolved on-chip delivery of compounds with a cellular resolution is demonstrated by chemical stimulation of patterned rat cortical neurons within a network with a number of solutions of excitatory neurotransmitter glutamate delivered via microfluidics. The combination of the system described with a patch-clamp technique allowed both modulation of the biochemical environment on a cellular level and the monitoring of electrophysiological properties in the reconstructed rat embryonic cortical networks changed by this microenvironment.
Authors:
Yulia Mourzina; Dmitry Kaliaguine; Petra Schulte; Andreas Offenhäusser
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Publication Detail:
Type:  Journal Article     Date:  2006-06-15
Journal Detail:
Title:  Analytica chimica acta     Volume:  575     ISSN:  1873-4324     ISO Abbreviation:  Anal. Chim. Acta     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2007-08-28     Completed Date:  2007-08-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370534     Medline TA:  Anal Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  281-9     Citation Subset:  -    
Affiliation:
Institute of Bio- and Nanosystems and Center of Nanoelectronic Systems for Information Technology, Research Center Jülich, 52425 Jülich, Germany. y.mourzina@fz-juelich.de
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