Document Detail

Pattern of growth and respiratory activity of Saccharomyces cerevisiae (baker's yeast) cells growing entrapped in an insolubilized gelatin gel.
MedLine Citation:
PMID:  8867890     Owner:  NLM     Status:  MEDLINE    
Yeast cells immobilized by entrapment in an insolubilized gelatin gel have been investigated with regard to their pattern of growth and respiratory activity. Mass-transfer resistance offered by the matrix and growth of the entrapped cells determine a gradient of nutrients throughout the gel which is responsible for both a lower specific growth rate of immobilized cells with respect to that of free ones, and a heterogeneous biomass distribution, with progressively increased cellular density from the inside to the outside of the matrix. The spatial organization of the growing cells leads to the formation of a biofilm perfectly adherent to the surface of the matrix. Measurements of the oxygen-uptake rate of the immobilized cells, after having been released from the matrix by trypsin digestion, and confocal microscopy of cells stained with Rhodamine 123 demonstrate the occurrence of a gradient of respiratory activity throughout the immobilized culture.
P Parascandola; E de Alteriis
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology and applied biochemistry     Volume:  23 ( Pt 1)     ISSN:  0885-4513     ISO Abbreviation:  Biotechnol. Appl. Biochem.     Publication Date:  1996 Feb 
Date Detail:
Created Date:  1996-12-30     Completed Date:  1996-12-30     Revised Date:  2007-03-21    
Medline Journal Info:
Nlm Unique ID:  8609465     Medline TA:  Biotechnol Appl Biochem     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  7-12     Citation Subset:  IM    
Dipartimento di Ingegneria Chimica e Alimentare, Università degli Studi di Salerno, Fisciano, Italy.
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MeSH Terms
Cell Division / physiology
Fluorescent Dyes
Microscopy, Confocal
Microscopy, Fluorescence
Oxygen Consumption / physiology*
Rhodamine 123
Saccharomyces cerevisiae / growth & development*,  physiology
Reg. No./Substance:
0/Fluorescent Dyes; 0/Gels; 0/Rhodamines; 62669-70-9/Rhodamine 123; 9000-70-8/Gelatin

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