Document Detail


Pattern of deposition of cell wall polysaccharides and transcript abundance of related cell wall synthesis genes during differentiation in barley endosperm.
MedLine Citation:
PMID:  22510768     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Immunolabeling, combined with chemical analyses and transcript profiling, have provided a comprehensive temporal and spatial picture of the deposition and modification of cell wall polysaccharides during barley (Hordeum vulgare) grain development, from endosperm cellularization at 3 d after pollination (DAP) through differentiation to the mature grain at 38 DAP. (1→3)-β-D-Glucan appears transiently during cellularization but reappears in patches in the subaleurone cell walls around 20 DAP. (1→3, 1→4)-β-Glucan, the most abundant polysaccharide of the mature barley grain, accumulates throughout development. Arabino-(1-4)-β-D-xylan is deposited significantly earlier than we previously reported. This was attributable to the initial deposition of the polysaccharide in a highly substituted form that was not recognized by antibodies commonly used to detect arabino-(1-4)-β-D-xylans in sections of plant material. The epitopes needed for antibody recognition were exposed by pretreatment of sections with α-L-arabinofuranosidase; this procedure showed that arabino-(1-4)-β-D-xylans were deposited as early as 5 DAP and highlighted their changing structures during endosperm development. By 28 DAP labeling of hetero-(1→4)-β-D-mannan is observed in the walls of the starchy endosperm but not in the aleurone walls. Although absent in mature endosperm cell walls we now show that xyloglucan is present transiently from 3 until about 6 DAP and disappears by 8 DAP. Quantitative reverse transcription-polymerase chain reaction of transcripts for GLUCAN SYNTHASE-LIKE, Cellulose Synthase, and CELLULOSE SYNTHASE-LIKE genes were consistent with the patterns of polysaccharide deposition. Transcript profiling of some members from the Carbohydrate-Active Enzymes database glycosyl transferase families GT61, GT47, and GT43, previously implicated in arabino-(1-4)-β-d-xylan biosynthesis, confirms their presence during grain development.
Authors:
Sarah M Wilson; Rachel A Burton; Helen M Collins; Monika S Doblin; Filomena A Pettolino; Neil Shirley; Geoffrey B Fincher; Antony Bacic
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-04-17
Journal Detail:
Title:  Plant physiology     Volume:  159     ISSN:  1532-2548     ISO Abbreviation:  Plant Physiol.     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-06-04     Completed Date:  2012-10-09     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  655-70     Citation Subset:  IM    
Affiliation:
Australian Research Council Centre of Excellence in Plant Cell Walls, School of Botany, University of Melbourne, Victoria 3010, Australia. wilsonsm@unimelb.edu.au
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MeSH Terms
Descriptor/Qualifier:
Cell Differentiation*
Cell Wall / genetics*,  metabolism
Endosperm / genetics,  metabolism*,  ultrastructure
Gene Expression Profiling / methods
Genes, Plant
Glucans / genetics,  metabolism
Glucosyltransferases / genetics,  metabolism
Hordeum / anatomy & histology,  genetics,  metabolism*
Immunohistochemistry
Microscopy, Electron, Transmission
Plant Cells / metabolism,  ultrastructure
Plant Proteins / genetics,  metabolism
Pollination
Polysaccharides / genetics,  metabolism*
RNA, Messenger / genetics,  metabolism
RNA, Plant / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Time Factors
Xylans / genetics,  metabolism
Chemical
Reg. No./Substance:
0/Glucans; 0/Plant Proteins; 0/Polysaccharides; 0/RNA, Messenger; 0/RNA, Plant; 0/Xylans; 37294-28-3/xyloglucan; 9040-27-1/arabinoxylan; 9064-51-1/callose; EC 2.4.1.-/Glucosyltransferases; EC 2.4.1.-/cellulose synthase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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