Document Detail


Patients with systemic lupus erythematosus are deficient in complement-dependent prevention of immune precipitation.
MedLine Citation:
PMID:  15054157     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: A functional deficiency of complement has been implicated but not conclusively demonstrated in the pathogenesis of systemic lupus erythematosus (SLE). To test this, we studied several aspects of complement in 44 patients with SLE, 46 patients with rheumatoid arthritis and 102 blood donors. METHODS: Prevention of immune precipitation (PIP) was measured by an enzyme immunoassay, levels of C1q, C4 and C3 by rocket immunoelectrophoresis, C4A, C4B and C3d by enzyme-linked immunosorbent assay (ELISA), complement haemolysis (CH50) by standard methods and C4 allotypes by high-voltage agarose electrophoresis and sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). RESULTS: PIP was significantly reduced in SLE (P<0.001); the defect was revealed by a sensitive assay measuring this function of complement but not by the other tests employed. The patients were clinically well at the time of study, and levels of C3d, which have been shown to correlate with disease activity, were normal. The defect was more common in patients with early disease (P = 0.009), supporting a role in aetiology or early pathophysiology. PIP was positively correlated with levels of C4 (P = 3 x 10(-5)) and in particular the C4A isotype (P = 9 x 10(-10)) whereas C4B was redundant. CONCLUSIONS: Our results reveal a defect in prevention of immune precipitation in SLE that is apparent at an early stage in the disease and correlates with low levels of C4A. These results indicate that subtle deficiencies of complement may predispose to SLE.
Authors:
G J Arason; K Steinsson; R Kolka; Th Víkingsdóttir; M S D'Ambrogio; H Valdimarsson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-03-30
Journal Detail:
Title:  Rheumatology (Oxford, England)     Volume:  43     ISSN:  1462-0324     ISO Abbreviation:  Rheumatology (Oxford)     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-05-27     Completed Date:  2004-07-13     Revised Date:  2007-09-06    
Medline Journal Info:
Nlm Unique ID:  100883501     Medline TA:  Rheumatology (Oxford)     Country:  England    
Other Details:
Languages:  eng     Pagination:  783-9     Citation Subset:  AIM; IM    
Affiliation:
Department of Immunology, Institute of Laboratory Medicine, Landspitalinn University Hospital, 101 Reykjavík, Iceland . garason@landspitali.is
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aged, 80 and over
Antigen-Antibody Complex / blood*
Arthritis, Rheumatoid / immunology
Autoimmune Diseases / immunology*
Complement C4a / deficiency
Complement Hemolytic Activity Assay
Complement System Proteins / analysis*,  immunology
Disease Progression
Female
Humans
Lupus Erythematosus, Systemic / immunology*
Male
Middle Aged
Chemical
Reg. No./Substance:
0/Antigen-Antibody Complex; 80295-49-4/Complement C4a; 9007-36-7/Complement System Proteins

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