Document Detail


Patients on chronic hemodialysis have no intrinsic lymphocyte defect upon stimulation with interleukin-2, interleukin-15 or tumor necrosis factor-alpha.
MedLine Citation:
PMID:  12601258     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Patients on chronic hemodialysis (HD) suffer from general immune incompetence, resulting in a high incidence of infectious complications, impaired response to vaccinations and a high incidence of malignancy. Although various abnormalities in T cell function of HD patients have been described, it remains unclear whether this is due to an intrinsic T cell defect. AIM: In the present study we tested the capacity of T cells to proliferate upon stimulation with antigen-presenting cell and T-cell-derived cytokines. METHODS: The proliferation capacity of lymphocytes obtained from patients on HD and healthy controls was determined by measuring the proliferation of peripheral blood mononuclear cells (PBMC) after stimulation with rhIL-2, rhIL-15, rhTNF-alpha, or combination of those cytokines. In all samples the percentage of alpha/beta TCR-positive T cells was measured. RESULTS: After isolation of PBMC the percentage of T cells varied from 70% (before stimulation) to 80% (after stimulation). IL-2, IL-15 and TNF-alpha all induced PBMC proliferation, while the combination TNF-alpha plus IL-2 or TNF-alpha plus IL-15 appeared to be additive. No difference between PBMC from HD patients and controls was found. CONCLUSION: We conclude that lymphocytes from HD patients have no intrinsic defects in their proliferation capacity after stimulation with IL-2, IL-15 or TNF-alpha, in vitro, as the increase in counts per minute is predominant.
Authors:
I C van Riemsdijk; C C Baan; E H M Loonen; R Zietse; W Weimar
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Blood purification     Volume:  21     ISSN:  0253-5068     ISO Abbreviation:  Blood Purif.     Publication Date:  2003  
Date Detail:
Created Date:  2003-02-25     Completed Date:  2003-10-03     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8402040     Medline TA:  Blood Purif     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  158-62     Citation Subset:  IM    
Copyright Information:
Copyright 2003 S. Karger AG, Basel
Affiliation:
Department of Internal Medicine, Erasmus University Medical Center Rotterdam, The Netherlands. vanriemsdijk@inw2.azr.nl
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MeSH Terms
Descriptor/Qualifier:
Cell Division / drug effects
Dose-Response Relationship, Drug
Drug Synergism
Female
Humans
Immunocompetence
Interleukin-15 / pharmacology*
Interleukin-2 / pharmacology*
Kidney Failure, Chronic / immunology,  therapy*
Lymphocyte Activation / drug effects*
Male
Middle Aged
Recombinant Proteins / pharmacology
Renal Dialysis*
T-Lymphocyte Subsets / drug effects*,  immunology
Tumor Necrosis Factor-alpha / pharmacology*
Chemical
Reg. No./Substance:
0/Interleukin-15; 0/Interleukin-2; 0/Recombinant Proteins; 0/Tumor Necrosis Factor-alpha

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