Document Detail


Patient-derived endothelial progenitor cells improve vascular graft patency in a rodent model.
MedLine Citation:
PMID:  21945828     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Late outgrowth endothelial progenitor cells (EPCs) derived from the peripheral blood of patients with significant coronary artery disease were sodded into the lumens of small diameter expanded polytetrafluoroethylene (ePTFE) vascular grafts. Grafts (1mm inner diameter) were denucleated and sodded either with native EPCs or with EPCs transfected with an adenoviral vector containing the gene for human thrombomodulin (EPC+AdTM). EPC+AdTM was shown to increase the in vitro rate of graft activated protein C (APC) production 4-fold over grafts sodded with untransfected EPCs (p<0.05). Unsodded control and EPC-sodded and EPC+AdTM-sodded grafts were implanted bilaterally into the femoral arteries of athymic rats for 7 or 28 days. Unsodded control grafts, both with and without denucleation treatment, each exhibited 7 day patency rates of 25%. Unsodded grafts showed extensive thrombosis and were not tested for patency over 28 days. In contrast, grafts sodded with untransfected EPCs or EPC+AdTM both had 7 day patency rates of 88-89% and 28 day patency rates of 75-88%. Intimal hyperplasia was observed near both the proximal and distal anastomoses in all sodded graft conditions but did not appear to be the primary occlusive failure event. This in vivo study suggests autologous EPCs derived from the peripheral blood of patients with coronary artery disease may improve the performance of synthetic vascular grafts, although no differences were observed between untransfected EPCs and TM transfected EPCs.
Authors:
J D Stroncek; L C Ren; B Klitzman; W M Reichert
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-09-06
Journal Detail:
Title:  Acta biomaterialia     Volume:  8     ISSN:  1878-7568     ISO Abbreviation:  Acta Biomater     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2011-11-29     Completed Date:  2012-03-19     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  101233144     Medline TA:  Acta Biomater     Country:  England    
Other Details:
Languages:  eng     Pagination:  201-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Acta Materialia Inc. All rights reserved.
Affiliation:
Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Vessel Prosthesis
Cells, Cultured
Endothelial Cells / cytology,  physiology*
Endothelium, Vascular / cytology*
Humans
Hyperplasia / pathology
Male
Protein C / metabolism
Rats
Rats, Nude
Stem Cells / cytology,  physiology*
Tunica Intima / pathology
Vascular Grafting / methods*
Vascular Patency*
Grant Support
ID/Acronym/Agency:
HL044972/HL/NHLBI NIH HHS; R01 HL044972-20/HL/NHLBI NIH HHS; T32 GM8555/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Protein C
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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