Document Detail


Pathways and mechanisms for product release in the engineered haloalkane dehalogenases explored using classical and random acceleration molecular dynamics simulations.
MedLine Citation:
PMID:  19577578     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Eight mutants of the DhaA haloalkane dehalogenase carrying mutations at the residues lining two tunnels, previously observed by protein X-ray crystallography, were constructed and biochemically characterized. The mutants showed distinct catalytic efficiencies with the halogenated substrate 1,2,3-trichloropropane. Release pathways for the two dehalogenation products, 2,3-dichloropropane-1-ol and the chloride ion, and exchange pathways for water molecules, were studied using classical and random acceleration molecular dynamics simulations. Five different pathways, denoted p1, p2a, p2b, p2c, and p3, were identified. The individual pathways showed differing selectivity for the products: the chloride ion releases solely through p1, whereas the alcohol releases through all five pathways. Water molecules play a crucial role for release of both products by breakage of their hydrogen-bonding interactions with the active-site residues and shielding the charged chloride ion during its passage through a hydrophobic tunnel. Exchange of the chloride ions, the alcohol product, and the waters between the buried active site and the bulk solvent can be realized by three different mechanisms: (i) passage through a permanent tunnel, (ii) passage through a transient tunnel, and (iii) migration through a protein matrix. We demonstrate that the accessibility of the pathways and the mechanisms of ligand exchange were modified by mutations. Insertion of bulky aromatic residues in the tunnel corresponding to pathway p1 leads to reduced accessibility to the ligands and a change in mechanism of opening from permanent to transient. We propose that engineering the accessibility of tunnels and the mechanisms of ligand exchange is a powerful strategy for modification of the functional properties of enzymes with buried active sites.
Authors:
Martin Klvana; Martina Pavlova; Tana Koudelakova; Radka Chaloupkova; Pavel Dvorak; Zbynek Prokop; Alena Stsiapanava; Michal Kuty; Ivana Kuta-Smatanova; Jan Dohnalek; Petr Kulhanek; Rebecca C Wade; Jiri Damborsky
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-07-03
Journal Detail:
Title:  Journal of molecular biology     Volume:  392     ISSN:  1089-8638     ISO Abbreviation:  J. Mol. Biol.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-09-21     Completed Date:  2009-10-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985088R     Medline TA:  J Mol Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1339-56     Citation Subset:  IM    
Affiliation:
Loschmidt Laboratories, Institute of Experimental Biology and National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Kamenice 5/A4, 625 00 Brno, Czech Republic.
Data Bank Information
Bank Name/Acc. No.:
PDB/3FBW;  3FWH;  3G9X
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MeSH Terms
Descriptor/Qualifier:
Alcohols / metabolism
Animals
Chlorides / metabolism
Hydrolases / chemistry*,  genetics,  metabolism*
Kinetics
Models, Chemical
Models, Molecular
Mutagenesis, Site-Directed
Propane / analogs & derivatives*,  metabolism
Protein Structure, Tertiary
Recombinant Proteins / chemistry,  genetics,  metabolism
Water / metabolism
Chemical
Reg. No./Substance:
0/Alcohols; 0/Chlorides; 0/Recombinant Proteins; 74-98-6/Propane; 7732-18-5/Water; 96-18-4/1,2,3-trichloropropane; EC 3.-/Hydrolases; EC 3.8.1.5/haloalkane dehalogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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