| Pathways for desaturation of oleoyl chains in Candida lipolytica. | |
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MedLine Citation:
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PMID: 6667424 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Incubation of microsomes from 25 degrees C- or 10 degrees C-grown cells of Candida lipolytica with [14C]oleoyl-CoA ( [14C]18:1-CoA) in the presence or absence of NADH resulted in rapid acyl transfer of [14C]18:1 to phospholipids (mainly phosphatidylcholine (PC) and phosphatidylethanolamine (PE) and to acylglycerols. Incorporation into PC was greatly enhanced when incubation was carried out in presence of lysophosphatidylcholine (lyso-PC). In all experiments, in the presence of NADH and O2, with and without added lyso-PC, the initial rate of formation of [14C]linoleoyl-PC was much greater than that of [14C]linoleoyl-CoA ( [14C]18:2-CoA). These results suggest that the actual substrate for the delta 12-desaturase is the oleoyl-PC, although some desaturation of 18:1-CoA cannot be eliminated. It is concluded that the main pathway for 18:2 formation proceeds from stearoyl-CoA (18:0-CoA) leads to 18:1-CoA leads to 18:1-phospholipid leads to 18:2-phospholipid; the pathway 18:0-CoA leads to 18:1-CoA leads to 18:2-CoA leads to 18:2-phospholipid is a minor pathway. Microsomes from cells grown at 10 degrees C had a higher content of 18:2 and a lower phospholipid desaturase activity at 25 degrees C than microsomes from cells grown at 25 degrees C, suggesting an inverse relationship between desaturase activity and membrane lipid fluidity. |
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Authors:
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G Ferrante; M Kates |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire Volume: 61 ISSN: 0714-7511 ISO Abbreviation: Can. J. Biochem. Cell Biol. Publication Date: 1983 Nov |
Date Detail:
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Created Date: 1984-03-27 Completed Date: 1984-03-27 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8302763 Medline TA: Can J Biochem Cell Biol Country: CANADA |
Other Details:
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Languages: eng Pagination: 1191-6 Citation Subset: IM |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acyl Coenzyme A
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metabolism* Acyltransferases / metabolism* Candida / metabolism* Fatty Acid Desaturases / metabolism* Microsomes / metabolism NAD / metabolism Phospholipids / biosynthesis |
| Chemical | |
Reg. No./Substance:
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0/Acyl Coenzyme A; 0/Phospholipids; 1716-06-9/oleoyl-coenzyme A; 53-84-9/NAD; EC 1.-/phospholipid desaturase; EC 1.14.19.-/Fatty Acid Desaturases; EC 1.14.99.-/delta-12 fatty acid desaturase; EC 2.3.-/Acyltransferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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