Document Detail


Pathways of assimilation of [13N]N2 and 13NH4+ by cyanobacteria with and without heterocysts.
MedLine Citation:
PMID:  418057     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The principal initial product of metabolism of [13N]N2 and 13NH4+ by five diverse cyanobacteria is glutamine. Methionine sulfoximine inhibits formation of [13N]glutamine except in the case of Gloeothece sp., an organism with a thick sheath through which the inhibitor may not penetrate. Thus, glutamine synthetase appears to catalyze the initial step in the assimilation of N2-derived or exogenous NH4+ by these organisms. [13N]Glutamate is, in all cases, the second major product of assimilation of 13N-labeled N2 and NH4+. In all of the N2-fixing cyanobacteria studied, the fraction of 13N in glutamine declines and that in glutamate increases with increasing times of assimilation of [13N]N2 and 13NH4+, and (Gloeothece again excepted) methionine sulfoximine reduces incorporation of 13N into glutamate as well as into glutamine. Glutamate synthase therefore appears to catalyze the formation of glutamate in a wide range of N2-fixing cyanobacteria. However, the major fraction of [13N]glutamate formed by Anacystis nidulans incubated with 13NH4+ may be formed by glutamic acid dehydrogenase. The formation of [13N]alanine from 13NH4+ appears to be catalyzed principally either by alanine dehydrogenase (as in Cylindrospermum licheniforme) or by a transaminase (as in Anabaena variabilis).
Authors:
J C Meeks; C P Wolk; W Lockau; N Schilling; P W Shaffer; W S Chien
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  134     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1978 Apr 
Date Detail:
Created Date:  1978-07-26     Completed Date:  1978-07-26     Revised Date:  2010-09-02    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  125-30     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Alanine / biosynthesis
Ammonia / metabolism*
Citrulline / biosynthesis
Cyanobacteria / metabolism*
Glutamates / biosynthesis
Glutamine / biosynthesis
Methionine Sulfoximine / pharmacology
Nitrogen Fixation*
Species Specificity
Chemical
Reg. No./Substance:
0/Glutamates; 1982-67-8/Methionine Sulfoximine; 372-75-8/Citrulline; 56-41-7/Alanine; 56-85-9/Glutamine; 7664-41-7/Ammonia
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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