| Pathways of assimilation of [13N]N2 and 13NH4+ by cyanobacteria with and without heterocysts. | |
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MedLine Citation:
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PMID: 418057 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The principal initial product of metabolism of [13N]N2 and 13NH4+ by five diverse cyanobacteria is glutamine. Methionine sulfoximine inhibits formation of [13N]glutamine except in the case of Gloeothece sp., an organism with a thick sheath through which the inhibitor may not penetrate. Thus, glutamine synthetase appears to catalyze the initial step in the assimilation of N2-derived or exogenous NH4+ by these organisms. [13N]Glutamate is, in all cases, the second major product of assimilation of 13N-labeled N2 and NH4+. In all of the N2-fixing cyanobacteria studied, the fraction of 13N in glutamine declines and that in glutamate increases with increasing times of assimilation of [13N]N2 and 13NH4+, and (Gloeothece again excepted) methionine sulfoximine reduces incorporation of 13N into glutamate as well as into glutamine. Glutamate synthase therefore appears to catalyze the formation of glutamate in a wide range of N2-fixing cyanobacteria. However, the major fraction of [13N]glutamate formed by Anacystis nidulans incubated with 13NH4+ may be formed by glutamic acid dehydrogenase. The formation of [13N]alanine from 13NH4+ appears to be catalyzed principally either by alanine dehydrogenase (as in Cylindrospermum licheniforme) or by a transaminase (as in Anabaena variabilis). |
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Authors:
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J C Meeks; C P Wolk; W Lockau; N Schilling; P W Shaffer; W S Chien |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Journal of bacteriology Volume: 134 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1978 Apr |
Date Detail:
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Created Date: 1978-07-26 Completed Date: 1978-07-26 Revised Date: 2010-09-02 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 125-30 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alanine
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biosynthesis Ammonia / metabolism* Citrulline / biosynthesis Cyanobacteria / metabolism* Glutamates / biosynthesis Glutamine / biosynthesis Methionine Sulfoximine / pharmacology Nitrogen Fixation* Species Specificity |
| Chemical | |
Reg. No./Substance:
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0/Glutamates; 1982-67-8/Methionine Sulfoximine; 372-75-8/Citrulline; 56-41-7/Alanine; 56-85-9/Glutamine; 7664-41-7/Ammonia |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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