Document Detail


Pathways affected by 3,5-diiodo-l-thyronine in liver of high fat-fed rats: evidence from two-dimensional electrophoresis, blue-native PAGE, and mass spectrometry.
MedLine Citation:
PMID:  20844788     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
3,5-Diiodo-l-thyronine (T2) powerfully reduces adiposity in rats fed a high-fat diet (HFD), stimulating (in the liver) fatty acid oxidation and mitochondrial uncoupling, and strongly counteracting steatosis, a condition commonly associated with diet-induced obesity. Proteomics offer unique possibilities for the investigation of changes in the levels and modifications of proteins. Here, combining 2D-E, mass spectrometry, and blue native (BN) PAGE, we studied how the subcellular hepatic phenotype responds to HFD and T2-treatment. By identifying differentially expressed proteins and analyzing their interrelation [using the Ingenuity Pathway Analysis (IPA) platform], we obtained an integrated view of the phenotypic/metabolic adaptations occurring in the liver proteome during HFD with or without T2-treatment. Interestingly, T2 counteracted several HFD-induced changes, mostly in mitochondria. BN-PAGE and subsequent in-gel activity analysis of OXPHOS complexes revealed a modified profile of individual complexes in HFD mitochondria vs. normal ones. This pattern was re-normalized in mitochondria from T2-treated HFD animals. These data indicate that in HFD rats, the effects of T2 on the liver proteome cause it to resemble that associated with a non-steatotic condition. The identified metabolic pathways (mainly at the mitochondrial level) may be responsible for the beneficial effects of T2 on liver adiposity and metabolism.
Authors:
Elena Silvestri; Federica Cioffi; Daniela Glinni; Michele Ceccarelli; Assunta Lombardi; Pieter de Lange; Angela Chambery; Valeria Severino; Antonia Lanni; Fernando Goglia; Maria Moreno
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-16
Journal Detail:
Title:  Molecular bioSystems     Volume:  6     ISSN:  1742-2051     ISO Abbreviation:  Mol Biosyst     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-11     Completed Date:  2011-01-24     Revised Date:  2011-05-16    
Medline Journal Info:
Nlm Unique ID:  101251620     Medline TA:  Mol Biosyst     Country:  England    
Other Details:
Languages:  eng     Pagination:  2256-71     Citation Subset:  IM    
Affiliation:
Dipartimento di Scienze Biologiche ed Ambientali, Università degli Studi del Sannio, Via Port'Arsa 11, 82100 Benevento, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
Computational Biology
Cytoplasm / metabolism
Dietary Fats / administration & dosage*,  pharmacology*
Diiodothyronines / administration & dosage,  pharmacology*
Electrophoresis, Gel, Two-Dimensional / methods*
Feeding Behavior / drug effects
Lipid Metabolism / drug effects
Liver / drug effects,  metabolism*
Mitochondria, Liver / drug effects,  metabolism
Mitochondrial Proteins / chemistry,  metabolism
Nuclear Proteins / chemistry,  metabolism
Proteomics
Rats
Rats, Wistar
Signal Transduction / drug effects*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Staining and Labeling
Triglycerides / metabolism
Chemical
Reg. No./Substance:
0/Dietary Fats; 0/Diiodothyronines; 0/Mitochondrial Proteins; 0/Nuclear Proteins; 0/Triglycerides; 534-51-0/3,5-diiodothyronine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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