Document Detail


The Rim101p/PacC pathway and alkaline pH regulate pattern formation in yeast colonies.
MedLine Citation:
PMID:  20038633     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Multicellular organisms utilize cell-to-cell signals to build patterns of cell types within embryos, but the ability of fungi to form organized communities has been largely unexplored. Here we report that colonies of the yeast Saccharomyces cerevisiae formed sharply divided layers of sporulating and nonsporulating cells. Sporulation initiated in the colony's interior, and this region expanded upward as the colony matured. Two key activators of sporulation, IME1 and IME2, were initially transcribed in overlapping regions of the colony, and this overlap corresponded to the initial sporulation region. The development of colony sporulation patterns depended on cell-to-cell signals, as demonstrated by chimeric colonies, which contain a mixture of two strains. One such signal is alkaline pH, mediated through the Rim101p/PacC pathway. Meiotic-arrest mutants that increased alkali production stimulated expression of an early meiotic gene in neighboring cells, whereas a mutant that decreased alkali production (cit1Delta) decreased this expression. Addition of alkali to colonies accelerated the expansion of the interior region of sporulation, whereas inactivation of the Rim101p pathway inhibited this expansion. Thus, the Rim101 pathway mediates colony patterning by responding to cell-to-cell pH signals. Cell-to-cell signals coupled with nutrient gradients may allow efficient spore formation and spore dispersal in natural environments.
Authors:
Sarah Piccirillo; Melissa G White; Jeffrey C Murphy; Douglas J Law; Saul M Honigberg
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2009-12-28
Journal Detail:
Title:  Genetics     Volume:  184     ISSN:  1943-2631     ISO Abbreviation:  Genetics     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-03-24     Completed Date:  2010-04-28     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  707-16     Citation Subset:  IM    
Affiliation:
Division of Cell Biology and Biophysics, School of Biological Sciences, University of Missouri, Kansas City, Missouri 64110, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Communication / physiology*
Hydrogen-Ion Concentration
Intracellular Signaling Peptides and Proteins / genetics,  metabolism
Meiosis / physiology
Nuclear Proteins / genetics,  metabolism
Protein-Serine-Threonine Kinases / genetics,  metabolism
Repressor Proteins / genetics,  metabolism*
Saccharomyces cerevisiae / physiology*
Saccharomyces cerevisiae Proteins / genetics,  metabolism*
Signal Transduction / physiology*
Spores, Fungal / genetics,  metabolism*
Transcription Factors / genetics,  metabolism
Transcription, Genetic / physiology*
Grant Support
ID/Acronym/Agency:
R15GM80710/GM/NIGMS NIH HHS; R15GM80710-S1/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/IME1 protein, S cerevisiae; 0/Intracellular Signaling Peptides and Proteins; 0/Nuclear Proteins; 0/RIM101 protein, S cerevisiae; 0/Repressor Proteins; 0/Saccharomyces cerevisiae Proteins; 0/Transcription Factors; EC 2.7.11.1/IME2 protein, S cerevisiae; EC 2.7.11.1/Protein-Serine-Threonine Kinases
Comments/Corrections

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