Document Detail


Pathotypes of diarrheagenic Escherichia coli in children attending a tertiary care hospital in South India.
MedLine Citation:
PMID:  20846583     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The prevalence of diarrheagenic Escherichia coli (DEC) in children under 5 years was studied in children with diarrhea and controls in South India. Four polymerase chain reaction (PCR) "schemes" were used to detect genes of the 6 pathotypes of DEC. In 394 children with diarrhea, 203 (52%) DEC infections were found. Among the 198 controls, 126 (63%) DEC infections were found. Enteroaggregative E. coli was the most common pathotype by multiplex PCR both in cases (58, 14.7%) and controls (47, 23.7%), followed by enteropathogenic E. coli seen in 10% cases and 8% of controls. Enterotoxigenic E. coli (ETEC), enterohemorrhagic E. coli (EHEC), enteroinvasive E. coli (EIEC), and diffusely adherent E. coli (DAEC) were found in 4.1%, 2.0%, 1.0%, and 0.5% of cases, respectively. ETEC was found in 2.5% of controls, but EHEC, EIEC, and DAEC were not detected. Overall, no single assay worked well, but by discounting genes with a pathogenicity index of less than 1, it was possible to use the PCR assays to identify DEC in 75/394 (19%) cases and 12/198 (6.1%) controls, while mixed infection could be identified in 8/394 (2%) cases and 2/198 (1%) controls.
Authors:
Priya Rajendran; Sitara Swarna Rao Ajjampur; Divya Chidambaram; Gunasekaran Chandrabose; Bhuvaneswari Thangaraj; Rajiv Sarkar; Prasanna Samuel; Deva Prasanna Rajan; Gagandeep Kang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Diagnostic microbiology and infectious disease     Volume:  68     ISSN:  1879-0070     ISO Abbreviation:  Diagn. Microbiol. Infect. Dis.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-17     Completed Date:  2011-01-25     Revised Date:  2014-02-27    
Medline Journal Info:
Nlm Unique ID:  8305899     Medline TA:  Diagn Microbiol Infect Dis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  117-22     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Child, Preschool
DNA, Bacterial / genetics
Diarrhea / epidemiology*,  microbiology*
Enterohemorrhagic Escherichia coli / classification,  genetics,  isolation & purification,  pathogenicity
Enteropathogenic Escherichia coli / classification,  genetics,  isolation & purification,  pathogenicity
Enterotoxigenic Escherichia coli / classification,  genetics,  isolation & purification,  pathogenicity
Escherichia coli / classification*,  genetics,  isolation & purification,  pathogenicity
Escherichia coli Infections / diagnosis,  epidemiology*,  microbiology*
Feces / microbiology
Genetic Variation
Humans
India
Infant
Infant, Newborn
Molecular Epidemiology
Polymerase Chain Reaction / methods
Prevalence
Grant Support
ID/Acronym/Agency:
D43 TW007392/TW/FIC NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Bacterial
Comments/Corrections

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