Document Detail


Pathophysiology of congenital diaphragmatic hernia II: the fetal lamb CDH model is surfactant deficient.
MedLine Citation:
PMID:  1501015     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The high mortality for congenital diaphragmatic hernia (CDH) has been attributed to a combination of pulmonary hypoplasia and pulmonary hypertension. We hypothesize that a surfactant deficiency may in part be contributing to the pathophysiology of CDH. This study documents the functional, quantitative, and qualitative aspects of the surfactant status of the alveolar air-liquid interface and the type II pneumocyte in the fetal lamb CDH model. Ten lamb fetuses (gestational age, 80 days) had a CDH created via a left thoracotomy and then were allowed to continue in utero development until term. Three litter mates and three nonoperated time-dated fetuses served as controls. At term, pressure-volume curves were performed to measure pulmonary compliance and total lung capacity. Alveolar lavage was then performed to measure the quantitative and the qualitative aspects of pulmonary surfactant. Finally, isolation of type II pneumocytes allowed quantification of phospholipid synthesis. When compared with controls (N = 6), the CDH lambs (N = 5) had significantly smaller lungs (P = .009), decreased total lung capacity (P less than .001) and compliance (P less than .001), reduced total lavaged phospholipids (P = .006), and decreased percent phosphatidylcholine (P = .02). CDH lambs also had increased total lavaged proteins (P = .05) and higher minimum dynamic surface tension (P less than .001). A surfactant deficiency may be contributing to the pathophysiology of CDH. Surfactant replacement therapy in premature infants has been shown to improve lung compliance, decrease morbidity, and improve survival. Exogenous surfactant may also benefit infants with CDH.
Authors:
P L Glick; V A Stannard; C L Leach; J Rossman; Y Hosada; F C Morin; D R Cooney; J E Allen; B Holm
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of pediatric surgery     Volume:  27     ISSN:  0022-3468     ISO Abbreviation:  J. Pediatr. Surg.     Publication Date:  1992 Mar 
Date Detail:
Created Date:  1992-09-17     Completed Date:  1992-09-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0052631     Medline TA:  J Pediatr Surg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  382-7; discussion 387-8     Citation Subset:  IM    
Affiliation:
Buffalo Institute of Fetal Therapy, Children's Hospital, NY 14222.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bronchoalveolar Lavage Fluid / chemistry
Embryonic and Fetal Development
Hernia, Diaphragmatic / congenital,  physiopathology*
Models, Biological
Phospholipids / analysis
Pulmonary Surfactants / analysis,  deficiency*
Respiratory Mechanics*
Sheep
Grant Support
ID/Acronym/Agency:
HL40896/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Phospholipids; 0/Pulmonary Surfactants
Comments/Corrections
Comment In:
J Pediatr Surg. 1993 Sep;28(9):1218-9   [PMID:  8308693 ]

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