Document Detail


Impact of endothelin A receptor antagonist selectivity in chronic nitric oxide synthase inhibition-induced fetal growth restriction in the rat.
MedLine Citation:
PMID:  20504166     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Endothelin receptor A (ETA) antagonism improves fetal and placental growth and placental perfusion on days 1 and 4, but not day 7 of a 7-day infusion of a nitric oxide synthase (NOS) inhibitor. Our purpose was to evaluate the significance of the degree of ETA antagonist selectivity on uteroplacental perfusion and fetal growth on day 7 of chronic NOS inhibition. METHODS: Timed-pregnant rats were treated with the NOS inhibitor nitro-L-arginine methyl ester (L-NAME, 2.5 mg/kg/h) with and without one of the following ETA antagonists or their respective vehicles for 7 days beginning on day 14 of gestation: A-127722 (2,000-fold selective for ETA over ETB), FR139317 (8,000-fold ETA-selective), or ABT-546 (28,000-fold ETA-selective). Uterine and placental perfusion, as well as fetal and placental weight, was evaluated at the 7th day of treatment (gestation day 21). RESULTS: L-NAME administration resulted in a significant reduction in uterine and placental perfusion as well as fetal and placental growth. In the setting of NOS inhibition, ETA antagonism did not improve uterine or placental perfusion or fetal growth after 7 days of infusion irrespective of the degree of selectivity of the antagonist used. CONCLUSIONS: ETA antagonism, irrespective of the degree of receptor selectivity, does not improve fetal growth or uteroplacental perfusion on day 7 of chronic NOS inhibition.
Authors:
Mark G Neerhof; Sylvia Synowiec; Saira Khan; Larry G Thaete
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Hypertension in pregnancy : official journal of the International Society for the Study of Hypertension in Pregnancy     Volume:  29     ISSN:  1525-6065     ISO Abbreviation:  Hypertens Pregnancy     Publication Date:  2010  
Date Detail:
Created Date:  2010-08-09     Completed Date:  2010-11-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9421297     Medline TA:  Hypertens Pregnancy     Country:  England    
Other Details:
Languages:  eng     Pagination:  284-93     Citation Subset:  IM    
Affiliation:
Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, North Shore University Health System, Evanston, Illinois 60201, USA. mneerhof@ enh.org
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Animals
Female
Fetal Growth Retardation / chemically induced,  metabolism*
Male
NG-Nitroarginine Methyl Ester / pharmacology
Nitric Oxide Synthase / antagonists & inhibitors*,  metabolism
Pregnancy
Rats
Rats, Sprague-Dawley
Receptors, Endothelin / antagonists & inhibitors*,  metabolism
Uterus / drug effects,  metabolism
Grant Support
ID/Acronym/Agency:
HD046968/HD/NICHD NIH HHS; R01 HD046968-05/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Receptors, Endothelin; 50903-99-6/NG-Nitroarginine Methyl Ester; EC 1.14.13.39/Nitric Oxide Synthase
Comments/Corrections

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