Document Detail

Pathologically elevated cyclic hydrostatic pressure induces CD95-mediated apoptotic cell death in vascular endothelial cells.
MedLine Citation:
PMID:  15772124     Owner:  NLM     Status:  MEDLINE    
We describe cyclic hydrostatic pressure of 200/100 mmHg with a frequency of 85/min as a hemodynamically relevant pathological condition enforcing apoptosis in endothelial cells (EC) after 24 h of treatment. This went along with an increase of CD95 and CD95L surface expression, shedding of CD95L into the supernatant, cleavage of caspase-3 and caspase-8, and elevated JNK-2, c-Jun, and CD95L mRNA expression. Furthermore, increased DNA-binding activity of the AP-1 transcription factor family members FRA-1 and c-Jun was observed. This activation was reduced by inhibition of JNK, which subsequently prevented elevated CD95L mRNA expression. Caspase inhibitors and a CD95L-neutralizing antibody also reduced EC apoptosis. Most of the pressure-induced events were most prominent at 24 and 48 h. However, after 48 h, the CD95/CD95L expression pattern switched back to CD95-/CD95L+ and the specific death rate decreased. Cyclic pathological hydrostatic pressure is a novel type of stress to EC that renders them susceptible to CD95/CD95L-mediated autoapoptosis and/or paracrine apoptosis accompanied by upregulation of intracellular molecules known to trigger both apoptosis and survival.
Cornelia Hasel; Susanne Dürr; Anke Bauer; Rene Heydrich; Silke Brüderlein; Tabe Tambi; Umesh Bhanot; Peter Möller
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-03-16
Journal Detail:
Title:  American journal of physiology. Cell physiology     Volume:  289     ISSN:  0363-6143     ISO Abbreviation:  Am. J. Physiol., Cell Physiol.     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-07-08     Completed Date:  2005-08-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100901225     Medline TA:  Am J Physiol Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  C312-22     Citation Subset:  IM    
Institute of Pathology, University of Ulm, Ulm, Germany.
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MeSH Terms
Antigens, CD95 / biosynthesis*
Aorta / cytology
Apoptosis / drug effects,  physiology*
Caspases / drug effects,  metabolism
Cells, Cultured
Electrophoretic Mobility Shift Assay
Endothelial Cells / metabolism,  pathology*
Enzyme Activation / physiology
Enzyme Inhibitors / pharmacology
Enzyme-Linked Immunosorbent Assay
Fas Ligand Protein
Flow Cytometry
Hydrostatic Pressure
Membrane Glycoproteins / biosynthesis
Reverse Transcriptase Polymerase Chain Reaction
Transcription Factors
Umbilical Veins / cytology
Reg. No./Substance:
0/Antigens, CD95; 0/Enzyme Inhibitors; 0/FASLG protein, human; 0/Fas Ligand Protein; 0/Membrane Glycoproteins; 0/Transcription Factors; EC 3.4.22.-/Caspases

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