Document Detail

Pathologic evaluation of normal and perfused term placental tissue.
MedLine Citation:
PMID:  24945897     Owner:  NLM     Status:  Publisher    
Abstract This study reports for the first time the incidence and interobserver variation of morphological findings in a series of 34 term placentas from pregnancies with normal outcome used for perfusion studies. Histologic evaluation of placental tissue is challenging, especially when it comes to defining "normal tissue" versus "pathological lesions". A scoring system for registration of abnormal morphological findings was developed. Light microscopic examination was performed independently by two pathologists, and inter-observer variation was analysed. Findings in normal and perfused tissue were compared and selected findings were tested against success parameters from the perfusions. Finally, the criteria for frequent lesions with fair to poor interobserver variation in the non-perfused tissue were revised and re-analysed. In the perfused tissue the perfusion artefact "trophoblastic vacuolization", which is believed to represent dilated transtrophoblastic channels, was reproducible and significantly correlated to the perfusion marker "fetal leakage". In longer perfusions, microscopy of the perfused cotelydon revealed bacteria in the fetal vessels. This finding led to an adjustment in the perfusion protocol with addition of antibiotics to the medium. In the "normal" tissue, certain lesions were very frequent and showed only fair or poor interobserver agreement. Revised minimum criteria for these lesions were defined and found reproducible. This study has emphasised the value of pathological examination as a supplement in placental perfusion models. Examination of the perfused cotelydon for trophoblastic vacuolization is recommended as an additional quality marker in perfusion models. The study also underlines the need for exact definitions of abnormality in frequent placental lesions.
Lisa Leth Maroun; Line Mathiesen; Morten Hedegaard; Lisbeth Ehlert Knudsen; Lise Grupe Larsen
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-6-19
Journal Detail:
Title:  Pediatric and developmental pathology : the official journal of the Society for Pediatric Pathology and the Paediatric Pathology Society     Volume:  -     ISSN:  1093-5266     ISO Abbreviation:  Pediatr. Dev. Pathol.     Publication Date:  2014 Jun 
Date Detail:
Created Date:  2014-6-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9809673     Medline TA:  Pediatr Dev Pathol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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