Document Detail

Pathogenicity of antigenic variants of murine coronavirus JHM selected with monoclonal antibodies.
MedLine Citation:
PMID:  3701929     Owner:  NLM     Status:  MEDLINE    
To analyze the pathogenesis of the neurotropic murine coronavirus JHMV, we used monoclonal antibodies to the E2 viral glycoprotein to select antigenic variant viruses. Monoclonal antibodies J.7.2 and J.2.2 were shown to bind to topographically distinct regions of the E2 molecule, and the variants selected with the two antibodies demonstrated very different disease pictures in mice. Variants selected with J.7.2 were, like the parental virus, highly virulent and caused an acute encephalitic illness. By contrast, J.2.2-selected variants predominantly caused a subacute paralytic disease clinically and extensive demyelination histologically. Antigenic differences among the variants and parental virus were readily demonstrable with anti-E2 monoclonal antibodies. However, no differences between the viruses could be shown in binding studies with monoclonal antibodies directed against either E1 or N, the other two JHMV structural proteins. Since only J.2.2 selected demyelinating variants with reduced neurovirulence, it is likely that this monoclonal antibody recognizes a subregion of the E2 molecule that is particularly important in JHMV pathogenesis.
J O Fleming; M D Trousdale; F A el-Zaatari; S A Stohlman; L P Weiner
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of virology     Volume:  58     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1986 Jun 
Date Detail:
Created Date:  1986-06-24     Completed Date:  1986-06-24     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  869-75     Citation Subset:  IM    
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MeSH Terms
Antibodies, Monoclonal / immunology*
Antigens, Viral / analysis*
Coronaviridae / growth & development,  immunology,  pathogenicity*
Demyelinating Diseases / etiology
Mice, Inbred C57BL
Paralysis / etiology
Virus Replication
Grant Support
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Viral

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