Document Detail


Pathogenicity of antigenic variants of murine coronavirus JHM selected with monoclonal antibodies.
MedLine Citation:
PMID:  3701929     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To analyze the pathogenesis of the neurotropic murine coronavirus JHMV, we used monoclonal antibodies to the E2 viral glycoprotein to select antigenic variant viruses. Monoclonal antibodies J.7.2 and J.2.2 were shown to bind to topographically distinct regions of the E2 molecule, and the variants selected with the two antibodies demonstrated very different disease pictures in mice. Variants selected with J.7.2 were, like the parental virus, highly virulent and caused an acute encephalitic illness. By contrast, J.2.2-selected variants predominantly caused a subacute paralytic disease clinically and extensive demyelination histologically. Antigenic differences among the variants and parental virus were readily demonstrable with anti-E2 monoclonal antibodies. However, no differences between the viruses could be shown in binding studies with monoclonal antibodies directed against either E1 or N, the other two JHMV structural proteins. Since only J.2.2 selected demyelinating variants with reduced neurovirulence, it is likely that this monoclonal antibody recognizes a subregion of the E2 molecule that is particularly important in JHMV pathogenesis.
Authors:
J O Fleming; M D Trousdale; F A el-Zaatari; S A Stohlman; L P Weiner
Related Documents :
1890189 - Immunologic characterization and specificity of three monoclonal antibodies against the...
3523489 - Production of a monoclonal antibody specific for seminomas and dysgerminomas.
8634489 - Preclinical evaluation of spect imaging with 131i-labeled monoclonal antibody sz39 in n...
8136179 - Murine hybridomas secreting monoclonal antibodies reacting with mlsa antigens.
22618529 - Interference in antiphospholipid antibody assays.
7503939 - Production and characterization of monoclonal antibodies recognising defined regions of...
21641419 - Highly sensitive detection of clenbuterol using competitive surface-enhanced raman scat...
8206429 - Polyspecific and autoreactive iga secreted by hybridomas derived from peyer's patches o...
6798889 - Cross-reactivity between trypanosoma cruzi and insect trypanosomatids as a basis for th...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of virology     Volume:  58     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1986 Jun 
Date Detail:
Created Date:  1986-06-24     Completed Date:  1986-06-24     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  869-75     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / immunology*
Antigens, Viral / analysis*
Coronaviridae / growth & development,  immunology,  pathogenicity*
Demyelinating Diseases / etiology
Male
Mice
Mice, Inbred C57BL
Paralysis / etiology
Virulence
Virus Replication
Grant Support
ID/Acronym/Agency:
NS00795/NS/NINDS NIH HHS; NS07149/NS/NINDS NIH HHS; NS18146/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Viral
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Evidence for H-2-linked control of retrovirus production in Friend virus-induced tumor cell lines.
Next Document:  Characterization of murine-specific leukemia virus receptor from L cells.