Document Detail


Pathogenicity of Salmonella: SopE-mediated membrane ruffling is independent of inositol phosphate signals.
MedLine Citation:
PMID:  16500648     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Studies [Zhou, D., Chen, L.-M., Hernandez, L., Shears, S.B., and Galán, J.E. (2001) A Salmonella inositol polyphosphatase acts in conjunction with other bacterial effectors to promote host-cell actin cytoskeleton rearrangements and bacterial internalization. Mol. Microbiol. 39, 248-259] with engineered Salmonella mutants showed that deletion of SopE attenuated the pathogen's ability to deplete host-cell InsP5 and remodel the cytoskeleton. We pursued these observations: In SopE-transfected host-cells, membrane ruffling was induced, but SopE did not dephosphorylate InsP5, nor did it recruit PTEN (a cytosolic InsP5 phosphatase) for this task. However, PTEN strengthened SopE-mediated membrane ruffling. We conclude SopE promotes host-cell InsP5 hydrolysis only with the assistance of other Salmonella proteins. Our demonstration that Salmonella-mediated cytoskeletal modifications are independent of inositolphosphates will focus future studies on elucidating alternate pathogenic consequences of InsP5 metabolism, including ion channel conductance and apoptosis.
Authors:
Sandrine Deleu; Kuicheon Choi; Jeff M Reece; Stephen B Shears
Related Documents :
11079768 - Maullinia ectocarpii gen. et sp. nov. (plasmodiophorea), an intracellular parasite in e...
12445638 - Antibacterial properties of cationic steroid antibiotics.
20442418 - In vivo identification of regulators of cell invasion across basement membranes.
16026808 - Comparative venom toxicity between pteromalus puparum and nasonia vitripennis (hymenopt...
8813148 - Expression of transduced carcinoembryonic antigen gene in human rhabdomyosarcoma inhibi...
3137178 - Spermatogenic onset. ii. fsh modulates mitotic activity of germ and sertoli cells in im...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural     Date:  2006-02-17
Journal Detail:
Title:  FEBS letters     Volume:  580     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-03-17     Completed Date:  2006-06-07     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1709-15     Citation Subset:  IM    
Affiliation:
Inositol Signaling Section, National Institute of Environmental Health Sciences, NIH, DHSS, Research Triangle Park, NC 27709, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acid Anhydride Hydrolases
Actins / metabolism
Bacterial Proteins / genetics,  physiology*
Cell Line, Tumor
Cell Membrane / microbiology*
Cytoskeleton / metabolism
Humans
Inositol Phosphates / physiology*
PTEN Phosphohydrolase / pharmacology
Salmonella / pathogenicity*
Salmonella Infections
Signal Transduction
Transfection
Virulence
Grant Support
ID/Acronym/Agency:
Z01 ES080046-19/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Actins; 0/Bacterial Proteins; 0/Inositol Phosphates; 0/SopE protein, Salmonella; 25663-09-6/inositol pentaphosphate; EC 3.1.3.67/PTEN Phosphohydrolase; EC 3.6.-/Acid Anhydride Hydrolases; EC 3.6.1.10/endopolyphosphatase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Cannabinoid derivatives induce cell death in pancreatic MIA PaCa-2 cells via a receptor-independent ...
Next Document:  The alkaline transition of blue copper proteins, Cucumis sativus plastocyanin and Pseudomonas aerugi...