Document Detail


Pathogenicity of Moraxella osloensis, a bacterium associated with the nematode Phasmarhabditis hermaphrodita, to the slug Deroceras reticulatum.
MedLine Citation:
PMID:  11679319     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Moraxella osloensis, a gram-negative bacterium, is associated with Phasmarhabditis hermaphrodita, a nematode parasite of slugs. This bacterium-feeding nematode has potential for the biological control of slugs, especially the grey garden slug, Deroceras reticulatum. Infective juveniles of P. hermaphrodita invade the shell cavity of the slug, develop into self-fertilizing hermaphrodites, and produce progeny, resulting in host death. However, the role of the associated bacterium in the pathogenicity of the nematode to the slug is unknown. We discovered that M. osloensis alone is pathogenic to D. reticulatum after injection into the shell cavity or hemocoel of the slug. The bacteria from 60-h cultures were more pathogenic than the bacteria from 40-h cultures, as indicated by the higher and more rapid mortality of the slugs injected with the former. Coinjection of penicillin and streptomycin with the 60-h bacterial culture reduced its pathogenicity to the slug. Further work suggested that the reduction and loss of pathogenicity of the aged infective juveniles of P. hermaphrodita to D. reticulatum result from the loss of M. osloensis from the aged nematodes. Also, axenic J1/J2 nematodes were nonpathogenic after injection into the shell cavity. Therefore, we conclude that the bacterium is the sole killing agent of D. reticulatum in the nematode-bacterium complex and that P. hermaphrodita acts only as a vector to transport the bacterium into the shell cavity of the slug. The identification of the toxic metabolites produced by M. osloensis is being pursued.
Authors:
L Tan; P S Grewal
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  67     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-10-26     Completed Date:  2002-01-17     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5010-6     Citation Subset:  IM    
Affiliation:
Department of Entomology, Ohio State University, Ohio Agricultural Research and Development Center, Wooster, Ohio 44691, USA. tan.100@osu.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Anti-Bacterial Agents / pharmacology
Colony Count, Microbial
Mollusca / microbiology*,  parasitology*
Moraxella / drug effects,  pathogenicity*,  physiology
Penicillins / pharmacology
Pest Control, Biological
Rhabditoidea / microbiology*,  physiology*
Streptomycin / pharmacology
Virulence
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Penicillins; 57-92-1/Streptomycin
Comments/Corrections

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