Document Detail

Pathogen transmission in child care settings studied by using a cauliflower virus DNA as a surrogate marker.
MedLine Citation:
PMID:  9534959     Owner:  NLM     Status:  MEDLINE    
Two regions of cauliflower mosaic virus DNA were designed as markers to study pathogen transmission in a child care home (CCH) and child care center (CCC) and in homes of CCC children. The DNA markers were stable for 1 month in the environment. The DNA markers were introduced into the environment through sensitized objects, and spread in the environment was traced by detection of the markers with polymerase chain reaction. The DNA markers spread rapidly in both the CCH and CCC after introduction and spread more rapidly in the toddler room than in the infant room of the CCC. Hand touching of contaminated areas was the major factor leading to spread of the markers. Hand washing and surface wiping decreased spread of the markers. The markers spread minimally from room to room in the CCC but were detected in the children's homes after introduction of markers in the CCC.
X Jiang; X Dai; S Goldblatt; C Buescher; T M Cusack; D O Matson; L K Pickering
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of infectious diseases     Volume:  177     ISSN:  0022-1899     ISO Abbreviation:  J. Infect. Dis.     Publication Date:  1998 Apr 
Date Detail:
Created Date:  1998-04-21     Completed Date:  1998-04-21     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0413675     Medline TA:  J Infect Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  881-8     Citation Subset:  AIM; IM    
Center for Pediatric Research, Children's Hospital of The King's Daughters, Eastern Virginia Medical School, Norfolk 23510-1001, USA.
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MeSH Terms
Biological Markers
Caulimovirus / genetics,  isolation & purification*
Child Care
Child Day Care Centers
Child, Preschool
DNA, Viral / isolation & purification*
Disease Transmission, Infectious
Environmental Microbiology
Gastroenteritis / virology*
Hand / virology
Polymerase Chain Reaction
Virus Diseases / transmission*
Reg. No./Substance:
0/Biological Markers; 0/DNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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