| Pathogen reduction treatment of buffy coat platelet concentrates in additive solution induces proapoptotic signaling. | |
| | |
MedLine Citation:
|
PMID: 22320126 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
BACKGROUND: Pathogen reduction technology (PRT) can potentially reduce the risk of transfusion-transmitted infections. However, PRT treatment of platelet (PLT) concentrates also results in reduced PLT quality and increased markers of apoptosis during storage. The aim of this study was to investigate changes to the expression and activation of proteins involved in apoptosis signaling. STUDY DESIGN AND METHODS: Samples from riboflavin and ultraviolet light PRT-treated and untreated (control) buffy coat-derived PCs in 70% SSP+ and 30% plasma were taken on Days 1, 5, and 7 of storage. Phosphatidylserine (PS) exposure, expression of Bcl-2 family proteins, cytochrome c release, and cleavage of caspase-3 and caspase-3 substrates were analyzed using flow cytometry and Western blotting. RESULTS: Compared to untreated controls, markers of apoptosis signaling were increased after PRT and subsequent storage. PS exposure on the PLT outer membrane was significantly higher after PRT on Days 5 and 7 of storage (p < 0.05). Expression of proapoptotic Bak and Bax was higher after PRT and subsequent storage. Cytochrome c release and caspase-3 cleavage were also greater and occurred earlier in the PRT-treated PLTs. The cleavage of caspase-3 substrates gelsolin and ROCK I were also increased after PRT, compared to untreated controls. CONCLUSIONS: This study demonstrated an increase in proapoptotic signaling during PLT storage, which was exacerbated by PRT. Many of these differences emerged outside the current 5-day storage period. These changes may not currently influence PLT transfusion quality, but will need to be carefully evaluated when considering extending PLT storage beyond 5 days. |
| | |
Authors:
|
Samantha Reid; Lacey Johnson; Narelle Woodland; Denese C Marks |
Related Documents
:
|
6766826 - Studies on the activities and properties of lysosomal hydrolases in fractionated popula... 3565536 - Electrical charge and joint inflammation. suppression of cationic absa-induced arthriti... 22498256 - Platelets enter atherosclerotic plaque via intraplaque microvascular leakage and intrap... 23652826 - The effects of ezetimibe/simvastatin versus simvastatin monotherapy on platelet and inf... 6860356 - Effects of media of low osmolarity on the dense bodies of human platelets. 12140466 - Evidence for pathogenic involvementof eosinophils and neutrophilsin churg-strauss syndr... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-2-10 |
Journal Detail:
|
Title: Transfusion Volume: - ISSN: 1537-2995 ISO Abbreviation: - Publication Date: 2012 Feb |
Date Detail:
|
Created Date: 2012-2-10 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0417360 Medline TA: Transfusion Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
© 2012 American Association of Blood Banks. |
Affiliation:
|
From Research and Development, Australian Red Cross Blood Service, and the School of Medical and Molecular Biosciences, University of Technology, Sydney, New South Wales, Australia. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Quantum Plasmonics: Nonlinear Effects in the Field Enhancement of a Plasmonic Nanoparticle Dimer.
Next Document: Maximum Principal Strain Correlates with Spinal Cord Tissue Damage in Contusion and Dislocation Inju...