Document Detail


Pathogen Detection Using a Liquid Array Technology*
MedLine Citation:
PMID:  21361936     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
  Low concentrations of microbial pathogens in pure and mixed samples were detected using a bead-based, liquid array technology. A 20-bp sequence in the 23S rRNA gene, rrl, was amplified in four microorganisms: Bacillus cereus, Escherichia coli, Salmonella enterica and Staphylococcus aureus. PCR products were positively identified with the Luminex(®) 100™ system. The system could detect very low amounts of DNA and the instrument response was proportional to the input concentration. The lower limit of detection (LLD) was determined to be 0.5 ng for B. cereus and E. coli and 2 ng for S. enterica. The LLD for S. aureus was not determined as the instrument response was still above the threshold when quantities of DNA as low as 0.25 ng were used. The platform positively identified organisms present in mixed samples even when the minor component was overshadowed by a 10-fold excess of the major component.
Authors:
Amanda Battaglia; Andrew J Schweighardt; Margaret M Wallace
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-3-1
Journal Detail:
Title:  Journal of forensic sciences     Volume:  -     ISSN:  1556-4029     ISO Abbreviation:  -     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-3-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375370     Medline TA:  J Forensic Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2011 American Academy of Forensic Sciences.
Affiliation:
Department of Sciences, John Jay College of Criminal Justice, City University of New York, 445 West 59th Street, New York, NY. Antech Diagnostics, Serology Department, 1111 Marcus Avenue, Lake Success, NY. Graduate School & University Center, City University of New York, 365 Fifth Avenue, New York, NY.
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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