| Paternal influence on S-phase in the first cell cycle of the bovine embryo. | |
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MedLine Citation:
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PMID: 7888500 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The objective of this study was to determine whether the initiation and length of the zygotic S-phase differs for embryos sired by bulls demonstrating high versus low fertility in vivo. Bovine oocytes were matured in vitro for 24-27 h and fertilized with frozen-thawed bovine semen. Six bulls that differed in fertility level were used in the study. The bulls were classified into two groups: those demonstrating high fertility in vivo (in vivo high-fertility bulls; n = 3), with a group mean +/- SEM lifetime nonreturn rate of 78 +/- 2%, and those demonstrating low fertility in vivo (low-fertility bulls; n = 3), with a group mean +/- SEM nonreturn rate of 69 +/- 1%. The S-phase in zygotes was identified by means of an immunocytochemical technique after pronuclear-stage zygotes were labeled with 5'bromo-2'deoxyuridine (BrdU). To visualize all pronuclei, presumptive zygotes were also stained with propidium iodide. In the first experiment, zygotes were labeled with BrdU at 2-h intervals from 8 to 20 h after sperm addition. There were no differences between bull fertility groups in the time course of pronuclear formation (p > 0.05). The beginning of S-phase was earlier in zygotes sired by high- compared to low-fertility bulls (p < 0.05). The end of S-phase was not affected by sire fertility group (p > 0.05). In the second experiment, zygotes were labeled with BrdU continuously from 8 to 20 h after sperm addition.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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L N Eid; S P Lorton; J J Parrish |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Biology of reproduction Volume: 51 ISSN: 0006-3363 ISO Abbreviation: Biol. Reprod. Publication Date: 1994 Dec |
Date Detail:
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Created Date: 1995-04-20 Completed Date: 1995-04-20 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0207224 Medline TA: Biol Reprod Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1232-7 Citation Subset: IM |
Affiliation:
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Department of Meat and Animal Science, University of Wisconsin-Madison 53706. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bromodeoxyuridine / metabolism Cattle / embryology* Cell Cycle / physiology Cell Division / physiology DNA / metabolism Embryo, Mammalian / cytology*, metabolism Embryonic and Fetal Development / physiology* Female Fertility / physiology Fertilization in Vitro Male Oocytes / physiology S Phase / physiology* Spermatozoa / physiology* Zygote / cytology, metabolism |
| Chemical | |
Reg. No./Substance:
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59-14-3/Bromodeoxyuridine; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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