Document Detail


Paternal influence on S-phase in the first cell cycle of the bovine embryo.
MedLine Citation:
PMID:  7888500     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objective of this study was to determine whether the initiation and length of the zygotic S-phase differs for embryos sired by bulls demonstrating high versus low fertility in vivo. Bovine oocytes were matured in vitro for 24-27 h and fertilized with frozen-thawed bovine semen. Six bulls that differed in fertility level were used in the study. The bulls were classified into two groups: those demonstrating high fertility in vivo (in vivo high-fertility bulls; n = 3), with a group mean +/- SEM lifetime nonreturn rate of 78 +/- 2%, and those demonstrating low fertility in vivo (low-fertility bulls; n = 3), with a group mean +/- SEM nonreturn rate of 69 +/- 1%. The S-phase in zygotes was identified by means of an immunocytochemical technique after pronuclear-stage zygotes were labeled with 5'bromo-2'deoxyuridine (BrdU). To visualize all pronuclei, presumptive zygotes were also stained with propidium iodide. In the first experiment, zygotes were labeled with BrdU at 2-h intervals from 8 to 20 h after sperm addition. There were no differences between bull fertility groups in the time course of pronuclear formation (p > 0.05). The beginning of S-phase was earlier in zygotes sired by high- compared to low-fertility bulls (p < 0.05). The end of S-phase was not affected by sire fertility group (p > 0.05). In the second experiment, zygotes were labeled with BrdU continuously from 8 to 20 h after sperm addition.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
L N Eid; S P Lorton; J J Parrish
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biology of reproduction     Volume:  51     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  1994 Dec 
Date Detail:
Created Date:  1995-04-20     Completed Date:  1995-04-20     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1232-7     Citation Subset:  IM    
Affiliation:
Department of Meat and Animal Science, University of Wisconsin-Madison 53706.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bromodeoxyuridine / metabolism
Cattle / embryology*
Cell Cycle / physiology
Cell Division / physiology
DNA / metabolism
Embryo, Mammalian / cytology*,  metabolism
Embryonic and Fetal Development / physiology*
Female
Fertility / physiology
Fertilization in Vitro
Male
Oocytes / physiology
S Phase / physiology*
Spermatozoa / physiology*
Zygote / cytology,  metabolism
Chemical
Reg. No./Substance:
59-14-3/Bromodeoxyuridine; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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