| Patch-clamp recording in myenteric neurons of guinea pig small intestine. | |
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MedLine Citation:
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PMID: 1616037 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The results of our research established the feasibility of applying patch-clamp methods in the study of the cellular neurophysiology of myenteric neurons enzymatically dissociated from adult guinea pig small intestine. Recording in current-clamp mode revealed two populations of neurons. One population discharged repetitively during depolarizing current pulses and displayed anodal-break excitation reminiscent of S/type 1 myenteric neurons. In the second population, spike discharge was limited to one or two spikes at the onset of depolarizing pulses and was similar to the behavior of AH/type 2 neurons. Recording in voltage-clamp mode revealed a complex of overlapping inward and outward whole cell currents. Fast and slow components of inward current were interpreted as sodium and calcium currents, respectively. Outward currents were blocked by cesium and consisted of components with properties of delayed rectifier current and A-type potassium current. |
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Authors:
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L V Baidan; A V Zholos; M F Shuba; J D Wood |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The American journal of physiology Volume: 262 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1992 Jun |
Date Detail:
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Created Date: 1992-07-29 Completed Date: 1992-07-29 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: G1074-8 Citation Subset: IM |
Affiliation:
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Department of Physiology, College of Medicine, Ohio State University, Columbus 43210-1238. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cesium / pharmacology Electric Stimulation Ganglia, Sympathetic / physiology* Guinea Pigs Intestine, Small / innervation* Membrane Potentials / drug effects Muscle, Smooth / innervation Myenteric Plexus / physiology* Neurons / drug effects, physiology* |
| Grant Support | |
ID/Acronym/Agency:
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R01-DK-37238/DK/NIDDK NIH HHS; R01-DK-39937/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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7440-46-2/Cesium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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