| The patatin-containing phospholipase A pPLAIIα modulates oxylipin formation and water loss in Arabidopsis thaliana. | |
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MedLine Citation:
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PMID: 22259021 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The patatin-related phospholipase A (pPLA) hydrolyzes membrane glycerolipids to produce monoacyl compounds and free fatty acids. Phospholipids are cleaved by pPLAIIα at the sn-1 and sn-2 positions, and galactolipids, including those containing oxophytodienoic acids, can also serve as substrates. Ablation of pPLAIIα decreased lysophosphatidylcholine and lysophosphatidylethanolamine levels, but increased free linolenic acid. pPLAIIα-deficient plants displayed a higher level of jasmonic acid and methyl jasmonate, as well as the oxylipin-biosynthetic intermediates 13-hydroperoxylinolenic acid and 12-oxophytodienoic acid than wild-type (WT) plants. The expression of genes involved in oxylipin production was also higher in the pPLAIIα-deficient mutant than in WT plants. The mutant plants lost water more quickly than WT plants. The stomata of WT and mutant plants responded similarly to abscisic acid. In response to desiccation, the mutant and WT leaves produced abscisic acid at the same rate, but, after 4 h of desiccation, the jasmonic acid level was much higher in mutant than WT leaves. These results indicate that pPLAIIα negatively regulates oxylipin production and suggest a role in the removal of oxidatively modified fatty acids from membranes. |
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Authors:
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Wen-Yu Yang; Yong Zheng; Sung Chul Bahn; Xiang-Qing Pan; Mao-Yin Li; Hieu Sy Vu; Mary R Roth; Brad Scheu; Ruth Welti; Yue-Yun Hong; Xue-Min Wang |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2012-01-18 |
Journal Detail:
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Title: Molecular plant Volume: 5 ISSN: 1752-9867 ISO Abbreviation: Mol Plant Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-03-23 Completed Date: 2012-07-17 Revised Date: 2013-04-15 |
Medline Journal Info:
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Nlm Unique ID: 101465514 Medline TA: Mol Plant Country: England |
Other Details:
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Languages: eng Pagination: 452-60 Citation Subset: IM |
Affiliation:
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Department of Biology, University of Missouri, St Louis, MO 63121, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Abscisic Acid
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pharmacology Arabidopsis / drug effects, enzymology*, genetics Arabidopsis Proteins / genetics, metabolism* Cyclopentanes / metabolism Droughts Galactolipids / metabolism Gene Expression Regulation, Plant / drug effects Gene Knockout Techniques Hydrolysis / drug effects Lysophospholipids / metabolism Oxidation-Reduction / drug effects Oxylipins / metabolism* Phenotype Phospholipases A / genetics, metabolism* Plant Leaves / drug effects, metabolism Plant Stomata / drug effects, physiology Substrate Specificity / drug effects Up-Regulation / drug effects Water / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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P20 RR-016475/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Arabidopsis Proteins; 0/Cyclopentanes; 0/Galactolipids; 0/Lysophospholipids; 0/Oxylipins; 0/pPLAIIalpha protein, Arabidopsis; 21293-29-8/Abscisic Acid; 6RI5N05OWW/jasmonic acid; 7732-18-5/Water; EC 3.1.1.-/Phospholipases A |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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