Document Detail


Pasteurella haemolytica ultraviolet-irradiated vaccine by parenteral and aerosol routes compared in the goat model.
MedLine Citation:
PMID:  9662614     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Positive control 1 (PC1) (n = 9) goats were injected transthoracically into the left lung with live Pasteurella haemolytica biovar A, serovar 1 (PhA1) in polyacrylate (PA) beads on days 0 and 21. Positive control 2 (PC2) (n = 6) goats were nebulized with live PhA1 and PA beads on days 0 and 21. Negative control (NC) goats (n = 6) were each injected transthoracically into the left lung with PA beads alone on days 0 and 21. Four groups (n = 6) were administered PA beads mixed with ultraviolet (UV) killed PhA1 on days 0 and 21. The treatment doses of bacteria for these groups were principal group 1 (PR1) injected into the left lung (7.7 x 10(10) cfu); PR2, 7.7 x 10(10) UV-killed PhA1 injected subcutaneously (SC); PR3, 7.7 x 10(10) UV-killed PhA1 injected SC only on day 21; PR4, nebulized with PA beads mixed with 5.6 x 10(10) cfu of UV-killed PhA1; and PR5, nebulized with PA beads mixed with 5 x 10(8) cfu of UV-killed PhA1. All goats were challenged transthoracically in the right lung with 1 x 10(8) cfu of live PhA1 on day 42 and necropsied on day 46. The sizes of consolidated lung lesions at the challenge site were used as a measure of immunity. The data show that the introduction of live PhA1 into the lungs of goats, either by injection or aerosolization, offers excellent protection against a subsequent homologous challenge. The data also demonstrate that two transthoracic injections (21 days apart) of UV-killed PhA1 (PR1), and subcutaneous injection of UV-killed PhA1(PR2) also offer excellent protection against a subsequent homologous live PhA1 challenge. One SC injection of UV-killed PhA1 (PR3) appears to offer only partial protection against a subsequent homologous live PhA1 challenge. Inhalation of UV-killed PhA1 mixed with PA beads (PR4 and PR5) induced no protection in goats against a subsequent live PhA1 transthoracic challenge.
Authors:
C W Purdy; J D Cooley; D C Straus
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Current microbiology     Volume:  37     ISSN:  0343-8651     ISO Abbreviation:  Curr. Microbiol.     Publication Date:  1998 Aug 
Date Detail:
Created Date:  1998-08-13     Completed Date:  1998-08-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7808448     Medline TA:  Curr Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  132-6     Citation Subset:  IM    
Affiliation:
Conservation and Production Research Laboratory, USDA Agriculture Research Service, Bushland, TX 79012, USA.
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MeSH Terms
Descriptor/Qualifier:
Aerosols
Animals
Bacterial Vaccines / administration & dosage*,  immunology,  therapeutic use
Body Temperature
Disease Models, Animal
Goats / immunology*
Infusions, Parenteral
Lung / immunology,  microbiology,  pathology
Lung Diseases / immunology,  microbiology,  prevention & control
Male
Mannheimia haemolytica / immunology*,  radiation effects*
Pasteurella Infections / blood
Rectum / immunology,  physiopathology
Ultraviolet Rays
Vaccines, Attenuated / administration & dosage,  immunology,  therapeutic use
Chemical
Reg. No./Substance:
0/Aerosols; 0/Bacterial Vaccines; 0/Vaccines, Attenuated

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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