| Passively released heme from hemoglobin and myoglobin is a potential source of nutrient iron for Bordetella bronchiseptica. | |
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MedLine Citation:
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PMID: 17664260 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Colonization by Bordetella bronchiseptica results in a variety of inflammatory respiratory infections, including canine kennel cough, porcine atrophic rhinitis, and a whooping cough-like disease in humans. For successful colonization, B. bronchiseptica must acquire iron (Fe) from the infected host. A vast amount of Fe within the host is sequestered within heme, a metalloporphyrin which is coordinately bound in hemoglobin and myoglobin. Utilization of hemoglobin and myoglobin as sources of nutrient Fe by B. bronchiseptica requires expression of BhuR, an outer membrane protein. We hypothesize that hemin is acquired by B. bronchiseptica in a BhuR-dependent manner after spontaneous loss of the metalloporphyrin from hemoglobin and/or myoglobin. Sequestration experiments demonstrated that direct contact with hemoglobin or myoglobin was not required to support growth of B. bronchiseptica in an Fe-limiting environment. Mutant myoglobins, each exhibiting a different affinity for heme, were employed to demonstrate that the rate of growth of B. bronchiseptica was directly correlated with the rate at which heme was lost from the hemoprotein. Finally, Escherichia coli cells expressing recombinant BhuR had the capacity to remove hemin from solution. Collectively, these experiments provided strong experimental support for the model that BhuR is a hemin receptor and B. bronchiseptica likely acquires heme during infection after passive loss of the metalloporphyrin from hemoglobin and/or myoglobin. These results also suggest that spontaneous hemin loss by hemoglobin and myoglobin may be a common mechanism by which many pathogenic bacteria acquire heme and heme-bound Fe. |
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Authors:
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Jeffrey C Mocny; John S Olson; Terry D Connell |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2007-07-30 |
Journal Detail:
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Title: Infection and immunity Volume: 75 ISSN: 0019-9567 ISO Abbreviation: Infect. Immun. Publication Date: 2007 Oct |
Date Detail:
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Created Date: 2007-09-20 Completed Date: 2007-10-31 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: United States |
Other Details:
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Languages: eng Pagination: 4857-66 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Immunology, The University at Buffalo, NY 14221, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bacterial Proteins / genetics, physiology Bordetella bronchiseptica / growth & development, metabolism* Escherichia coli / genetics, metabolism Hemin / metabolism* Hemoglobins / metabolism* Humans Iron / metabolism Myoglobin / metabolism* Receptors, Cell Surface / genetics, physiology Sperm Whale |
| Grant Support | |
ID/Acronym/Agency:
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DE007034/DE/NIDCR NIH HHS; GM35649/GM/NIGMS NIH HHS; HL47020/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Hemoglobins; 0/Myoglobin; 0/Receptors, Cell Surface; 16009-13-5/Hemin; 7439-89-6/Iron |
| Comments/Corrections | |
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