| Passive recruitment of circulating leukocytes into capillary sprouts from existing capillaries in a microfluidic system. | |
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MedLine Citation:
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PMID: 21503282 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Recent evidence implicating leukocytes in angiogenesis raises the question of whether leukocytes and other cells circulating with the blood in microvascular networks can home to capillary sprouts intraluminally. This study describes an investigation of leukocyte trafficking in sprouting capillaries fabricated using soft lithography. The leukocytes passing with whole blood through existing capillaries were able to enter microfabricated capillary sprouts of variable length and sprouting angle due to the mechanical interaction with red blood cells (RBCs) at the sprouting bifurcation, in spite of the complete absence of blood flow through the blind-ended sprouts or any chemoattractants. The RBCs formed "comet tails" (the densely packed cellular trains forming behind leukocytes as they move through narrow capillaries) and effectively pushed leukocytes into the microfabricated sprouts while bypassing them at the sprouting bifurcation. Individual sprouts filled with several leukocytes, as wells as RBCs and platelets, were observed. The results of this study suggest that (i) blood cells are likely present in capillary sprouts throughout their development, (ii) leukocytes and other circulating cells may use this mechanism to home to capillary sprouts intraluminally for direct engraftment, and (iii) tissues may use this phenomenon as another mechanism for local recruitment of leukocytes from the blood stream. |
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Authors:
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Omid Forouzan; Jennie M Burns; Jennifer L Robichaux; Walter L Murfee; Sergey S Shevkoplyas |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-4-18 |
Journal Detail:
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Title: Lab on a chip Volume: - ISSN: 1473-0189 ISO Abbreviation: - Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-4-19 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101128948 Medline TA: Lab Chip Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Biomedical Engineering, Tulane University, New Orleans, LA 70118, USA. shevkop@tulane.edu. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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