| Passive immunization with a polyclonal antiserum to the hemoglobin receptor of Haemophilus ducreyi confers protection against a homologous challenge in the experimental swine model of chancroid. | |
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MedLine Citation:
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PMID: 21646451 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Haemophilus ducreyi, the etiologic agent of chancroid, has an obligate requirement for heme. Heme is acquired by H. ducreyi from its human host via TonB-dependent transporters expressed at its bacterial surface. Of 3 TonB-dependent transporters encoded in the genome of H. ducreyi, only the hemoglobin receptor, HgbA, is required to establish infection during the early stages of the experimental human model of chancroid. Active immunization with a native preparation of HgbA (nHgbA) confers complete protection in the experimental swine model of chancroid, using either Freund's or monophosphoryl lipid A as adjuvants. To determine if transfer of anti-nHgbA serum is sufficient to confer protection, a passive immunization experiment using pooled nHgbA antiserum was conducted in the experimental swine model of chancroid. Pigs receiving this pooled nHgbA antiserum were protected from a homologous, but not a heterologous, challenge. Passively transferred polyclonal antibodies elicited to nHgbA bound the surface of H. ducreyi and partially blocked hemoglobin binding by nHgbA, but were not bactericidal. Taken together, these data suggest that the humoral immune response to the HgbA vaccine is protective against an H. ducreyi infection, possibly by preventing acquisition of the essential nutrient heme. |
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Authors:
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Isabelle Leduc; William G Fusco; Neelima Choudhary; Patty A Routh; Deborah M Cholon; Marcia M Hobbs; Glen W Almond; Paul E Orndorff; Christopher Elkins |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2011-06-06 |
Journal Detail:
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Title: Infection and immunity Volume: 79 ISSN: 1098-5522 ISO Abbreviation: Infect. Immun. Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-07-18 Completed Date: 2011-09-13 Revised Date: 2012-02-01 |
Medline Journal Info:
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Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: United States |
Other Details:
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Languages: eng Pagination: 3168-77 Citation Subset: IM |
Affiliation:
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Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. isabelle_leduc@med.unc.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Bacterial / administration & dosage*, immunology Bacterial Proteins / immunology* Carrier Proteins / immunology* Chancroid / immunology, pathology, prevention & control* Disease Models, Animal Ear / pathology Haemophilus ducreyi / immunology, pathogenicity* Histocytochemistry Immune Sera / administration & dosage*, immunology Immunization, Passive / methods* Microbial Viability Microscopy Receptors, Cell Surface / immunology Swine Swine Diseases / immunology, prevention & control |
| Grant Support | |
ID/Acronym/Agency:
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5-R01-AI 05393/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Bacterial; 0/Bacterial Proteins; 0/Carrier Proteins; 0/Immune Sera; 0/Receptors, Cell Surface; 0/hemoglobin-binding protein, bacteria |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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