Document Detail

Passive gravitational sedimentation of peripheral blood increases the sensitivity of microscopic detection of malaria.
MedLine Citation:
PMID:  23768828     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To determine if passive gravitational sedimentation of blood samples, followed by buffy coat thin smear preparation could increase the sensitivity of malaria diagnosis when compared to conventional thin smear preparation without the additional cost of centrifuges or molecular diagnostics.
METHODS: Blood samples were collected from 205 patients. Each patient sample was analyzed using all three methods of sample preparation.
RESULTS: Buffy coat analysis of centrifuged blood samples greatly increased the sensitivity of malaria diagnosis when compared to standard thin smear techniques. Sensitivity between mechanically centrifuged samples and gravitationally sedimented samples showed equal improvement in sensitivity when compared to standard thin smear preparation.
CONCLUSIONS: Passive gravitational sedimentation of red blood cells followed by buffy coat analysis dramatically improves the sensitivity of malaria diagnosis without the additional costs associated with centrifugation.
Richard Davis; Trenden Flanigan; Eric Wilson
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Multicenter Study    
Journal Detail:
Title:  Asian Pacific journal of tropical medicine     Volume:  6     ISSN:  1995-7645     ISO Abbreviation:  Asian Pac J Trop Med     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-06-17     Completed Date:  2013-10-17     Revised Date:  2014-04-08    
Medline Journal Info:
Nlm Unique ID:  101533720     Medline TA:  Asian Pac J Trop Med     Country:  Singapore    
Other Details:
Languages:  eng     Pagination:  552-5     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Hainan Medical College. Published by Elsevier B.V. All rights reserved.
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MeSH Terms
Blood Buffy Coat
Blood Sedimentation
Erythrocytes / parasitology
Feasibility Studies
Malaria / diagnosis*
Sensitivity and Specificity

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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