| Passive administration of antibody against retinal S-antigen induces electroretinographic supernormality. | |
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MedLine Citation:
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PMID: 1730546 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Electroretinographic supernormality, affecting both the a- and b-waves of the electroretinogram (ERG), occurs consistently before the onset of experimental autoimmune uveoretinitis (EAU) in rabbits and rats. To investigate the possible role of antibody to S-antigen (S-ab) in this phenomenon, affinity-purified polyclonal rat S-ab was injected intravenously into normal rats and administered to isolated rat eyecup preparations by bolus perfusion. In both situations, ERG supernormality was observed. The effect in vivo peaked 90 min after injection, and ERG changes in vitro were observed within 15 sec. The ERG response in vivo and in vitro was dose dependent and was abolished in vivo by pretreatment with cyproheptadine (a serotonin antagonist). The ERG was not affected in either system by a control rat antibody (antiovalbumin) or by murine monoclonal or rabbit polyclonal antibodies to S-antigen. The induction of ERG supernormality in vivo and in vitro by homologous S-ab indicates the operation of species-specific mechanisms both involving and bypassing the blood-retinal barrier and highlights a significant role for humoral autoimmunity in the pathogenesis of S-antigen-induced EAU in the rat. |
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Authors:
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M R Stanford; J Robbins; E Kasp; D C Dumonde |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Investigative ophthalmology & visual science Volume: 33 ISSN: 0146-0404 ISO Abbreviation: Invest. Ophthalmol. Vis. Sci. Publication Date: 1992 Jan |
Date Detail:
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Created Date: 1992-02-20 Completed Date: 1992-02-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7703701 Medline TA: Invest Ophthalmol Vis Sci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 30-5 Citation Subset: IM |
Affiliation:
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Department of Immunology, UMDS, St. Thomas' Hospital, London, England. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens / immunology* Arrestin Autoantibodies / administration & dosage* Autoantigens / immunology* Cattle Chromatography, Affinity Cyproheptadine / administration & dosage Electrophoresis, Polyacrylamide Gel Electroretinography* Eye Proteins / immunology* Injections, Intravenous Rabbits Rats Retinitis / immunology, physiopathology* Uveitis / immunology, physiopathology* |
| Chemical | |
Reg. No./Substance:
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0/Antigens; 0/Arrestin; 0/Autoantibodies; 0/Autoantigens; 0/Eye Proteins; 129-03-3/Cyproheptadine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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