Document Detail


Passive pressure-diameter relationship and structural composition of rat mesenteric lymphangions.
MedLine Citation:
PMID:  23145980     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Lymph flow depends on both the rate of lymph production by tissues and the extent of passive and active pumping. Here we aim to characterize the passive mechanical properties of a lymphangion in both mid-lymphangion and valve segments to assess regional differences along a lymphangion, as well as evaluating its structural composition.
METHODS AND RESULTS: Mesenteric lymphatic vessels were isolated and cannulated in a microchamber for pressure-diameter (P-D) testing. Vessels were inflated from 0 to 20 cmH(2)O at a rate of 4 cmH(2)O/min, and vessel diameter was continuously tracked, using an inverted microscope, video camera, and custom LabVIEW program, at both mid-lymphangion and valve segments. Isolated lymphatic vessels were also pressure-fixed at 2 and 7 cmH(2)O and imaged using a nonlinear optical microscope (NLOM) to obtain collagen and elastin structural information. We observed a highly nonlinear P-D response at low pressures (3-5 cmH(2)O), which was modeled using a three-parameter constitutive equation. No significant difference in the passive P-D response was observed between mid-lymphangion and valve regions. NLOM imaging revealed an inner elastin layer and outer collagen layer at all locations. Lymphatic valve leaflets were predominantly elastin with thick axially oriented collagen bands at the insertion points.
CONCLUSIONS: We observed a highly nonlinear P-D response at low pressures (3-5 cmH(2)O) and developed the first constitutive equation to describe the passive P-D response for a lymphangion. The passive P-D response did not vary among regions, in agreement with the composition of elastin and collagen in the lymphatic wall.
Authors:
Elaheh Rahbar; Jon Weimer; Holly Gibbs; Alvin T Yeh; Christopher D Bertram; Michael J Davis; Michael A Hill; David C Zawieja; James E Moore
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, N.I.H., Extramural     Date:  2012-11-12
Journal Detail:
Title:  Lymphatic research and biology     Volume:  10     ISSN:  1557-8585     ISO Abbreviation:  Lymphat Res Biol     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-17     Completed Date:  2013-05-23     Revised Date:  2013-12-04    
Medline Journal Info:
Nlm Unique ID:  101163587     Medline TA:  Lymphat Res Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  152-63     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Algorithms
Animals
Collagen / metabolism
Elastin / metabolism
Histocytochemistry
Lymphatic Vessels / anatomy & histology*,  metabolism,  physiology*
Male
Mesentery
Microscopy, Confocal
Models, Biological*
Pressure
Rats
Rats, Sprague-Dawley
Grant Support
ID/Acronym/Agency:
HL-089784/HL/NHLBI NIH HHS; HL070308/HL/NHLBI NIH HHS; R01 HL094269/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
9007-34-5/Collagen; 9007-58-3/Elastin
Comments/Corrections

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