Document Detail


Passage in mice causes a change in the ability of Salmonella enterica serovar Oranienburg to survive NaCl osmotic stress: resuscitation from the viable but non-culturable state.
MedLine Citation:
PMID:  12076792     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An outbreak caused by dried processed squids contaminated with Salmonella Oranienburg occurred in Japan in 1999. Isolates obtained from the causative food were resistant to NaCl osmotic stress, but isolates from the patients were sensitive to NaCl. Although strains from both sources were almost identical in their virulence in mice, a NaCl-resistant strain from food (Sa9911T) became NaCl-sensitive after passage in mice and a NaCl-sensitive strain from one patient (Sa99004) retained NaCl sensitivity after such passage. When dried squid was contaminated experimentally with both strains during processing, only Sa9911T was recovered directly from the final product. Nevertheless, the viability of the Sa99004 cells was over 90% found by fluorescent staining. We suggested that Sa99004 might become viable but non-culturable (VNC) by NaCl stress. This hypothesis was confirmed by resuscitation by efficient enrichment. We concluded that VNC S. Oranienburg would be potentially dangerous contaminants of NaCl-preserved foods and that measures to ensure their detection should be taken at the time of food inspection.
Authors:
Hiroshi Asakura; Sou-ichi Makino; Tsuyoshi Takagi; Asumi Kuri; Takayuki Kurazono; Masahisa Watarai; Toshikazu Shirahata
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS microbiology letters     Volume:  212     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-06-21     Completed Date:  2002-09-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  87-93     Citation Subset:  IM    
Affiliation:
Department of Veterinary Microbiology, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Culture Media
DNA, Bacterial / analysis
Decapodiformes / microbiology*
Desiccation
Disease Outbreaks
Drug Resistance, Bacterial
Food Handling
Food Microbiology
Humans
Male
Mice
Mice, Inbred BALB C
Osmotic Pressure
Salmonella Food Poisoning / epidemiology,  microbiology*
Salmonella Infections, Animal / microbiology,  physiopathology
Salmonella enterica / growth & development*,  isolation & purification,  pathogenicity,  physiology
Serial Passage*
Sodium Chloride / pharmacology*
Chemical
Reg. No./Substance:
0/Culture Media; 0/DNA, Bacterial; 7647-14-5/Sodium Chloride

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