Document Detail


Parturition at term: parallel increases in interleukin-8 and proteinase concentrations and neutrophil count in the lower uterine segment.
MedLine Citation:
PMID:  10221247     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A relationship was sought between the tissue concentrations of interleukin (IL)-8, matrix metalloproteinase (MMP)-8 and MMP-9, and the numbers of the various leukocytes infiltrating the lower uterine segment stroma during parturition. Biopsy specimens of the lower uterine segment were obtained from 63 women undergoing Caesarean section at various stages of cervical dilatation at term. The concentrations of IL-8, MMP-8 and MMP-9 were determined with enzyme-linked immunosorbent assays, and the leukocytes were quantified immunohistochemically. The median IL-8 concentration (pg/mg total protein) rose significantly from 17.2 at < 2 cm dilatation, to 26.5 at 2 to < 4 cm dilatation, and 1954.0 at 4-6 cm dilatation, and remained at approximately this concentration at > 6 cm dilatation. The median MMP-8 concentration (ng/mg total protein) increased significantly from 32.2 at < 2 cm dilatation to 114.2 at > 6 cm dilatation. The median MMP-9 concentration (ng/mg total protein) rose significantly from 15.4 at < 2 cm dilatation to 102.1 at > 6 cm dilatation. The number of neutrophils was significantly higher at 4-6 cm and > 6 cm dilatation than at > 2 cm, reaching maximum values at > 6 cm dilatation. The findings in this study support the hypothesis that IL-8-induced infiltration of the cervical stroma by neutrophils and subsequent release of proteinases may play a key role in parturition.
Authors:
M Winkler; D C Fischer; P Ruck; T Marx; E Kaiserling; A Oberpichler; H Tschesche; W Rath
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  14     ISSN:  0268-1161     ISO Abbreviation:  Hum. Reprod.     Publication Date:  1999 Apr 
Date Detail:
Created Date:  1999-06-22     Completed Date:  1999-06-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1096-100     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynaecology, University Hospital, Technical University (RWTH), Aachen, Germany.
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MeSH Terms
Descriptor/Qualifier:
Adult
Collagenases / physiology*
Female
Humans
Interleukin-8 / physiology*
Labor, Obstetric / physiology*
Leukocyte Count
Matrix Metalloproteinase 8
Matrix Metalloproteinase 9
Neutrophils / pathology,  physiology*
Pregnancy
Uterus / physiology*
Chemical
Reg. No./Substance:
0/Interleukin-8; EC 3.4.24.-/Collagenases; EC 3.4.24.34/Matrix Metalloproteinase 8; EC 3.4.24.35/Matrix Metalloproteinase 9

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