| Partitioning of new carbon as ¹¹C in Nicotiana tabacum reveals insight into methyl jasmonate induced changes in metabolism. | |
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MedLine Citation:
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PMID: 20842413 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We examined the timeline by which methyl jasmonate (MeJA) reprograms new carbon partitioning into key metabolite pools. The radioactive isotope ¹¹C (t(¹/₂) 20.4 min), administered to intact leaves of Nicotiana tabacum L. (cv Samsun) as ¹¹CO(2) gas enabled us to measure changes in new carbon partitioning into soluble sugar and amino acid pools of [¹¹C]photosynthate. A 500 μM MeJA treatment resulted in a decrease in the [¹¹C]soluble sugar pool and an increase in the [¹¹C]amino acid pool after 4 h. This pattern was more pronounced 15 h after treatment. We also examined the timeline for ¹¹C-partitioning into aromatic amino acid metabolites of the shikimate pathway. [¹¹C]Tyrosine, [C¹¹C]phenylalanine and [¹¹C]tryptophan were elevated 1.5-fold, 12-fold and 12-fold, respectively, relative to controls, 4 h after MeJA treatment, while endogeneous pools were unchanged. This suggests that only new carbon is utilized during early stages of defense induction. By 15 h, [C¹¹C]tyrosine and [¹¹C]phenylalanine returned to baseline while [¹¹C]tryptophan was elevated 30-fold, suggesting that MeJA exerts selective control over the shikimate pathway. Finally, we measured trans-cinnamic acid levels as a gauge of downstream phenolic metabolism. Levels were unchanged 4 h after MeJA treatment relative to controls, but were increased 2-fold by 15 h, indicating a lag in response of secondary metabolism. |
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Authors:
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Nils Hanik; Sara Gómez; Marcel Best; Michael Schueller; Colin M Orians; Richard A Ferrieri |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-09-15 |
Journal Detail:
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Title: Journal of chemical ecology Volume: 36 ISSN: 1573-1561 ISO Abbreviation: J. Chem. Ecol. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-10-11 Completed Date: 2011-01-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7505563 Medline TA: J Chem Ecol Country: United States |
Other Details:
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Languages: eng Pagination: 1058-67 Citation Subset: IM |
Affiliation:
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Fachbereich Chemie, Johannes Gutenberg Universität, 55099, Mainz, Germany. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetic Acids
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pharmacology Carbon Isotopes / metabolism* Cyclopentanes / pharmacology* Ecosystem Oxylipins / pharmacology Phenols / metabolism Phenylalanine / metabolism Plant Growth Regulators / pharmacology* Plant Leaves / drug effects, metabolism Shikimic Acid / metabolism Time Factors Tobacco / drug effects*, metabolism Tryptophan / metabolism Tyrosine / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Acetic Acids; 0/Carbon Isotopes; 0/Cyclopentanes; 0/Oxylipins; 0/Phenols; 0/Plant Growth Regulators; 1211-29-6/methyl jasmonate; 138-59-0/Shikimic Acid; 55520-40-6/Tyrosine; 63-91-2/Phenylalanine; 73-22-3/Tryptophan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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