Document Detail

Particle-based synthesis of peptide arrays.
MedLine Citation:
PMID:  19191248     Owner:  NLM     Status:  MEDLINE    
Lithographic methods allow for the combinatorial synthesis of >50,000 oligonucleotides per cm(2), and this has revolutionized the field of genomics. High-density peptide arrays promise to advance the field of proteomics in a similar way, but currently lag behind. This is mainly due to the monomer-by-monomer repeated consecutive coupling of 20 different amino acids associated with lithography, which adds up to an excessive number of coupling cycles. Combinatorial synthesis based on electrically charged solid amino acid particles resolves this problem. A color laser printer or a chip addresses the different charged particles consecutively to a solid support, where, when completed, the whole layer of solid amino acid particles is melted at once. This frees hitherto immobilized amino acids to couple all 20 different amino acids to the support in one single coupling reaction. The method should allow for the translation of entire genomes into sets of overlapping peptides to be used in proteome research.
Frank Breitling; Thomas Felgenhauer; Alexander Nesterov; Volker Lindenstruth; Volker Stadler; F Ralf Bischoff
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chembiochem : a European journal of chemical biology     Volume:  10     ISSN:  1439-7633     ISO Abbreviation:  Chembiochem     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-30     Completed Date:  2009-06-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100937360     Medline TA:  Chembiochem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  803-8     Citation Subset:  IM    
Department of Chip-Based Peptide Arrays, German Cancer Research Center, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany.
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MeSH Terms
Amino Acids / chemistry*
Microarray Analysis* / instrumentation,  methods
Particle Size
Peptides / chemical synthesis*,  chemistry
Reg. No./Substance:
0/Amino Acids; 0/Peptides

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