| Participation of reactive oxygen species in PGF2alpha-induced apoptosis in rat luteal cells. | |
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MedLine Citation:
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PMID: 11058439 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Prostaglandin F(2alpha) (PGF(2alpha)) is implicated in the process of luteal regression in many species. Treatment of rat luteal tissue with PGF(2alpha) increases the generation of reactive oxygen species. Since reactive oxygen species have been implicated in apoptosis, the present study was undertaken to determine whether reactive oxygen species play a role in the PGF(2alpha)-induced apoptosis of rat luteal cells. Rat luteal cells were loaded with 6-carboxy-2, 7'-dichlorodihydro-fluorescein (CDCFH) diacetate, di (acetomethyl ester), which can be oxidized by reactive oxygen species to yield CDCF, a fluorescent molecule, and the cells were treated with different doses of PGF(2alpha). Incubation with 100 micromol PGF(2alpha) l(-1) induced an increase in CDCF fluorescence (P < 0. 05). Treatment of cells with PGF(2alpha) for 48 h in serum-free medium induced a dose-dependent increase in cell death, and these cells exhibited the morphological characteristics typical of apoptosis, including condensed or fragmented nuclei and fragmentation of internucleosomal DNA. Pretreatment of these cells with ascorbic acid, N,N'-dimethylthiourea, or superoxide dismutase, which acts as an antioxidant or a radical scavenger, prevented the PGF(2alpha)-induced apoptosis. These results demonstrate that PGF(2alpha) produces reactive oxygen species and induces apoptosis in rat luteal cells, indicating that the reactive oxygen species may induce apoptotic cell death during luteolysis. |
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Authors:
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M Tanaka; T Miyazaki; S Tanigaki; K Kasai; K Minegishi; K Miyakoshi; H Ishimoto; Y Yoshimura |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of reproduction and fertility Volume: 120 ISSN: 0022-4251 ISO Abbreviation: J. Reprod. Fertil. Publication Date: 2000 Nov |
Date Detail:
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Created Date: 2001-01-25 Completed Date: 2001-01-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376367 Medline TA: J Reprod Fertil Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 239-45 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, Keio University, School of Medicine, 35 Shinanomachi, Shinjuku, Tokyo 160-8582, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Analysis of Variance Animals Antioxidants / pharmacology Apoptosis* Ascorbic Acid / pharmacology Cells, Cultured Corpus Luteum / drug effects, metabolism DNA Fragmentation Dinoprostone / pharmacology* Female Free Radical Scavengers / pharmacology Least-Squares Analysis Luteolysis / drug effects* Microscopy, Fluorescence Rats Rats, Sprague-Dawley Reactive Oxygen Species / metabolism* Superoxide Dismutase / pharmacology Thiourea / analogs & derivatives, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Free Radical Scavengers; 0/Reactive Oxygen Species; 363-24-6/Dinoprostone; 50-81-7/Ascorbic Acid; 61805-96-7/1,3-dimethylthiourea; 62-56-6/Thiourea; EC 1.15.1.1/Superoxide Dismutase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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