| Participation of oxygen in the bacterial transformation of 2,4,6-trinitrotoluene. | |
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MedLine Citation:
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PMID: 18457577 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The exposure of Bacillus cereus ZS18 cell suspensions to 2,4,6-trinitrotoluene (TNT) in the absence of other oxidizable substrates increases oxygen uptake, exceeding the basal level of respiration of the bacterium 1.5- and 2-fold with 50 and 100 mg/liter of TNT, respectively. The interaction of both living and to less extent dead bacterial cells with TNT results in the accumulation of superoxide anion (O2*-) in the extracellular medium, which was revealed by the EPR spectroscopy. The accumulation of O2*- decreased by 50-70% in the presence of Cu,Zn-superoxide dismutase of animal origin. In the presence of living bacterial cells, the level of TNT decreased progressively, yielding hydroxylaminodinitrotoluenes together with O2*-. In the presence of heat-killed cells, a moderate decrease in TNT was observed, and the appearance of O2*- was not accompanied by the production of any detectable TNT metabolites. Chelating agents inhibited the transformation of TNT and decreased the formation of O2*-. The demonstrated generation of O2*- during the interaction of TNT with K4[Fe(CN)6] together with the observed effects of chelating agents suggest the participation of iron in the one-electron reduction of TNT and the functioning of an extracellular redox cycle with the involvement of molecular oxygen. |
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Authors:
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E A Naumenko; A V Naumov; E S Suvorova; R Gerlach; A M Ziganshin; A P Lozhkin; N I Silkin; R P Naumova |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochemistry. Biokhimii͡a Volume: 73 ISSN: 0006-2979 ISO Abbreviation: Biochemistry Mosc. Publication Date: 2008 Apr |
Date Detail:
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Created Date: 2008-05-06 Completed Date: 2008-09-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376536 Medline TA: Biochemistry (Mosc) Country: United States |
Other Details:
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Languages: eng Pagination: 463-9 Citation Subset: IM |
Affiliation:
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Kazan State University, Kazan 420008, Russia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aerobiosis Bacillus cereus / metabolism* Oxidation-Reduction Oxygen / metabolism Superoxides / metabolism* Trinitrotoluene / chemistry*, metabolism |
| Chemical | |
Reg. No./Substance:
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11062-77-4/Superoxides; 118-96-7/Trinitrotoluene; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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