Document Detail

Participation of concanavalin A binding sites in the interaction between Trypanosoma cruzi and macrophages.
MedLine Citation:
PMID:  6352717     Owner:  NLM     Status:  MEDLINE    
Untreated mouse peritoneal macrophages as well as macrophages treated with concanavalin A (ConA) were incubated in the presence of untreated or ConA-treated epimastigotes and trypomastigotes of Trypanosoma cruzi. Treatment of epimastigotes or trypomastigotes with ConA increased or decreased their uptake by macrophages, respectively. Treatment of their macrophages with ConA reduced by 70% and increased by five times the ingestion of epimastigotes and trypomastigotes, respectively. These results are discussed in relation to previous studies on the mobility of ConA receptors in the membrane of the parasite. Using fluorescein- or ferritin-labelled ConA we observed that ConA binding sites located on the plasma membrane of macrophages are internalized during endocytosis of T. cruzi, and observed in association with the membrane of the endocytic vacuole. Vacuoles without parasites showed a uniform distribution of ConA binding sites, while these sites were distributed in patches in vacuoles containing parasites. These results, in association with others previously reported, suggest the involvement of glycoproteins and/or glycolipids localized on the cell surface of T. cruzi and macrophages during the T. cruzi-macrophage interaction.
M N Meirelles; A Martinez-Palomo; T Souto-Padron; W De Souza
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cell science     Volume:  62     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  1983 Jul 
Date Detail:
Created Date:  1983-11-23     Completed Date:  1983-11-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  287-99     Citation Subset:  IM    
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MeSH Terms
Cell Communication / drug effects
Cell Membrane / ultrastructure
Concanavalin A / pharmacology
Macrophages / physiology*,  ultrastructure
Microscopy, Electron
Receptors, Concanavalin A / physiology*
Trypanosoma cruzi / drug effects,  physiology*
Reg. No./Substance:
0/Receptors, Concanavalin A; 11028-71-0/Concanavalin A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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