Document Detail

Participation of cathepsins B and D in apoptosis of PC12 cells following serum deprivation.
MedLine Citation:
PMID:  9790930     Owner:  NLM     Status:  MEDLINE    
Cathepsin D, a lysosomal aspartic proteinase, has been shown to induce apoptosis of HeLa cells when overexpressed. To further understand regulatory mechanisms of cathepsin D-induced cell death, we examined whether lysosomal cysteine and aspartic proteinases are involved in apoptosis of PC12 cells following serum deprivation. In serum deprived culture, PC12 cells overexpressing cathepsin D died more rapidly than wild-type cells. When the active forms of cathepsins B and D were examined during the apoptotic process of wild-type cells, the amount of cathepsin B was drastically reduced 24 hr after the onset of culture, whereas that of cathepsin D considerably increased. The viability of PC12 cells overexpressing cathepsin B was significantly higher in serum-deprived culture than wild-type cells. In this situation, the amount of the cathepsin B protein did not decrease. The results suggest that there exists an apoptotic pathway regulated by lysosomal cathepsins B and D.
M Shibata; S Kanamori; K Isahara; Y Ohsawa; A Konishi; S Kametaka; T Watanabe; S Ebisu; K Ishido; E Kominami; Y Uchiyama
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  251     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1998 Oct 
Date Detail:
Created Date:  1998-11-23     Completed Date:  1998-11-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  199-203     Citation Subset:  IM    
Copyright Information:
Copyright 1998 Academic Press.
Department of Cell Biology and Anatomy I, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan.
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MeSH Terms
Apoptosis / drug effects,  physiology*
Cathepsin B / physiology*
Cathepsin D / physiology*
Cell Survival / drug effects
Cells, Cultured
Culture Media, Serum-Free / pharmacology
In Situ Nick-End Labeling
Lysosomes / enzymology
Nerve Growth Factors / pharmacology
PC12 Cells / cytology,  drug effects,  enzymology*
Reg. No./Substance:
0/Culture Media, Serum-Free; 0/Nerve Growth Factors; EC B; EC D

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