| Participation of adrenomedullin and its relation with vascular endothelial growth factor in androgen regulation of prostatic blood flow in vivo. | |
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MedLine Citation:
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PMID: 17113911 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: We had previously reported that androgen-regulated prostatic blood flow and vascular endothelial growth factor (VEGF) were involved in the signal transduction pathway. Adrenomedullin (ADM) is a multifunctional regulatory peptide with mitogenic and angiogenic capabilities that are regulated by androgen. ADM is abundantly expressed in the prostate. We focused on ADM and evaluated its participation and relation with VEGF in androgen prostatic blood flow regulation using a castrated rat model. METHODS: We examined the effect of locally injected dihydrotestosterone (DHT) and ADM, and the co-administration of DHT with an ADM receptor antagonist (ADM 22-52) on prostatic blood flow. Furthermore, prostatic blood flow was evaluated after ADM and VEGF administration with each other's antagonist, VEGF neutralizing antibody and ADM 22-52, respectively. Changes in the mRNA expression levels of ADM in the prostate after castration and successive androgen stimulation were also evaluated. RESULTS: The administration of ADM promptly increased prostatic blood flow in a dose-dependent manner within 30 minutes. The DHT-induced increase in prostatic blood flow was completely abolished by co-administration with anti-ADM. Anti-ADM inhibited the VEGF-induced prostatic blood flow elevation, but a VEGF neutralizing antibody did not affect the ADM-mediated blood flow elevation. Furthermore, upregulation of the ADM gene induced by DHT was inhibited by co-administration with a VEGF-neutralizing antibody. CONCLUSIONS: These results have clearly demonstrated the direct regulation of prostatic blood flow by ADM and its involvement in androgenic prostatic blood flow regulation. Furthermore, ADM was estimated to be a downstream mediator of VEGF action in the signal transduction pathway. |
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Authors:
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Yasuhiro Shibata; Bunzo Kashiwagi; Seiji Arai; Tomohiro Magari; Kazuhiro Suzuki; Seijiro Honma |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Urology Volume: 68 ISSN: 1527-9995 ISO Abbreviation: Urology Publication Date: 2006 Nov |
Date Detail:
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Created Date: 2006-11-20 Completed Date: 2006-12-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0366151 Medline TA: Urology Country: United States |
Other Details:
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Languages: eng Pagination: 1127-31 Citation Subset: IM |
Affiliation:
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Department of Urology, Gunma University Graduate School of Medicine, Gunma, Japan. yshibata@med.gunma-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adrenomedullin
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physiology* Androgens / physiology* Animals Male Prostate / blood supply* Rats Rats, Wistar Regional Blood Flow / physiology Vascular Endothelial Growth Factor A / physiology* |
| Chemical | |
Reg. No./Substance:
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0/Androgens; 0/Vascular Endothelial Growth Factor A; 148498-78-6/Adrenomedullin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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