Document Detail


Participation of translesion synthesis DNA polymerases in the maintenance of chromosome integrity in yeast Saccharomyces cerevisiae.
MedLine Citation:
PMID:  21568839     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We employed a genetic assay based on illegitimate hybridization of heterothallic Saccharomyces cerevisiae strains (the α-test) to analyze the consequences for genome stability of inactivating translesion synthesis (TLS) DNA polymerases. The α-test is the only assay that measures the frequency of different types of mutational changes (point mutations, recombination, chromosome or chromosome arm loss) and temporary changes in genetic material simultaneously. All these events are manifested as illegitimate hybridization and can be distinguished by genetic analysis of the hybrids and cytoductants. We studied the effect of Polζ, Polη, and Rev1 deficiency on the genome stability in the absence of genotoxic treatment and in UV-irradiated cells. We show that, in spite of the increased percent of accurately repaired primary lesions, chromosome fragility, rearrangements, and loss occur in the absence of Polζ and Polη. Our findings contribute to further refinement of the current models of translesion synthesis and the organization of eukaryotic replication fork.
Authors:
O V Kochenova; J V Soshkina; E I Stepchenkova; S G Inge-Vechtomov; P V Shcherbakova
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemistry. Biokhimii͡a     Volume:  76     ISSN:  1608-3040     ISO Abbreviation:  Biochemistry Mosc.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-05-16     Completed Date:  2011-09-19     Revised Date:  2014-09-15    
Medline Journal Info:
Nlm Unique ID:  0376536     Medline TA:  Biochemistry (Mosc)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  49-60     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Chromosomes, Fungal / genetics*,  metabolism,  radiation effects
DNA-Directed DNA Polymerase / genetics,  metabolism*
Genomic Instability / radiation effects
Mutation / radiation effects
Saccharomyces cerevisiae / enzymology*,  genetics,  metabolism,  radiation effects
Saccharomyces cerevisiae Proteins / genetics,  metabolism*
Ultraviolet Rays
Grant Support
ID/Acronym/Agency:
ES011644/ES/NIEHS NIH HHS; ES015869/ES/NIEHS NIH HHS; K22 ES011644/ES/NIEHS NIH HHS; K22 ES011644-02/ES/NIEHS NIH HHS; R01 ES015869/ES/NIEHS NIH HHS; R01 ES015869-02/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Saccharomyces cerevisiae Proteins; EC 2.7.7.7/DNA-Directed DNA Polymerase
Comments/Corrections

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