Document Detail

Participation of the Ca(2+)-calmodulin-activated Kinase II in the control of metaphase-anaphase transition in human cells.
MedLine Citation:
PMID:  11401327     Owner:  NLM     Status:  MEDLINE    
Treatment of HeLa S3 cells growing in suspension, and of endothelial cells ECV 304 growing as a monolayer, with the inhibitor of the Ca(2+)-calmodulin activated Kinase II KN-93, blocks cells at metaphase for 15 min (HeLa cells) and 30 min (ECV cells). Thereafter cells resume mitosis and enter anaphase. The inactive isomer KN-92 does not show such effects. The results show the involvement of the CaM K II system in the regulation of the metaphase-anaphase transition whereby the activation of the Kinase II, in particular by calmodulin appears to be affected, the residual autophosphorylation of the CaM K II system apparently sufficing after 15 to 30 min to release the cells into anaphase. The results are compared with the metaphase-blocking effects of the noble gas xenon, where the xenon-induced block can be overcome by small intracellular increases of Ca(2+), thus indicating the CaM K II system as a possible target for xenon.
C P Petzelt; S Kodirov; G Taschenberger; W J Kox
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biology international     Volume:  25     ISSN:  1065-6995     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2001  
Date Detail:
Created Date:  2001-06-12     Completed Date:  2001-08-02     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  403-9     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
Experimental Anesthesiology, Department of Anesthesiology and Intensive Care, University Hospital Charité, Humboldt-University, Spandauer Damm 130, Berlin, D-14050, Germany.
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MeSH Terms
Anaphase / physiology*
Benzylamines / pharmacology
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Calcium-Calmodulin-Dependent Protein Kinases / drug effects,  metabolism*
Dose-Response Relationship, Drug
Enzyme Inhibitors / pharmacology
Hela Cells
Metaphase / physiology*
Sulfonamides / pharmacology
Xenon / pharmacology
Reg. No./Substance:
0/Benzylamines; 0/Enzyme Inhibitors; 0/Sulfonamides; 139298-40-1/KN 93; 7440-63-3/Xenon; EC Protein Kinase Type 2; EC Protein Kinases

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