| Partial purification and functional properties of an endoprotease from bovine neurosecretory granules cleaving proocytocin/neurophysin peptides at the basic amino acid doublet. | |
| | |
MedLine Citation:
|
PMID: 2825769 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
An enriched preparation of neurosecretory granules from bovine pituitary neural lobes was used as a source of processing enzymes possibly involved in the cleavage of the proocytocin/neurophysin precursor. A synthetic eicosapeptide reproducing the entire (1-20) sequence of the NH2-terminal domain of the bovine ocytocin/neurophysin precursor was used as a substrate to monitor an endoprotease activity cleaving at the Lys11-Arg12 doublet. The 58-kDa endoprotease detected in the lysate of neurohypophyseal granules produced a single cleavage, after the doublet, at the Arg12-Ala13 peptide bond. This endoprotease with pHi 6.9 and 7.2 exhibits maximal activity at pH around neutrality (7.0) and was strongly inhibited by divalent cation chelating agents [ethylenediaminetetraacetic acid and ethylene glycol bis(beta-aminoethyl ether)-N,N,N',-N'-tetraacetic acid] and to some extent by p-(chloromercuri)benzoate and p-(chloromercuri)benzenesulfonic acid, while phenylmethanesulfonyl fluoride and pepstatin were not active. This endoprotease action was sensitive to any modification of the substrate at either basic amino acid of the doublet since replacement of either L-Lys11 or L-Arg12 by D-Lys or D-Arg and by L-Nle abolished the cleavage reaction. In contrast, reversal of the polarity of the doublet in [Arg11,Lys12]proocytocin/neurophysin(1-20) had no effect on the mode of endoproteolytic cleavage as well as modifications of Gly10 (replaced by Ala10). It is concluded that the selectivity of this endoprotease, which may be involved in the primary event occurring in proocytocin/neurophysin processing, is strictly dependent upon the integrity of the basic doublet but that other parameters determined by the amino acid sequence around this doublet may play an important role. |
| | |
Authors:
|
C Clamagirand; C Creminon; C Fahy; H Boussetta; P Cohen |
Related Documents
:
|
2696959 - High sequence conservation between isocitrate lyase from escherichia coli and ricinus c... 21540299 - Metabolic cycles in a circannual hibernator. 10679199 - Molecular characterization reveals identity of microtubule-associated proteins map3 and... 6089869 - Studies on adenosine triphosphate transphosphorylases. amino acid sequence of rabbit mu... 6279639 - Ovalbumin utilizes an nh2-terminal signal sequence. 23220819 - Nitrogen removal of ramie stalk treated by acid wastewater combined with clostridium th... 7806149 - The role of sodium in the uptake of ursodeoxycholic acid in isolated hamster hepatocytes. 2845839 - Arachidonic acid metabolites produced by bovine alveolar macrophages. 15271989 - Loss of cell wall mannosylphosphate in candida albicans does not influence macrophage r... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biochemistry Volume: 26 ISSN: 0006-2960 ISO Abbreviation: Biochemistry Publication Date: 1987 Sep |
Date Detail:
|
Created Date: 1988-02-02 Completed Date: 1988-02-02 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0370623 Medline TA: Biochemistry Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 6018-23 Citation Subset: IM |
Affiliation:
|
Groupe de Neurobiochimie Cellulaire et Moléculaire, Université Pierre et Marie Curie, Paris, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Animals Cattle Cell Fractionation Centrifugation, Density Gradient Cytoplasmic Granules / enzymology* Kinetics Neurophysins* Oxytocin* Peptide Hydrolases / isolation & purification*, metabolism Pituitary Gland, Posterior / enzymology* Povidone Protein Precursors* Silicon Dioxide Substrate Specificity |
| Chemical | |
Reg. No./Substance:
|
0/Neurophysins; 0/Protein Precursors; 50-56-6/Oxytocin; 65455-52-9/Percoll; 7631-86-9/Silicon Dioxide; 9003-39-8/Povidone; EC 3.4.-/Peptide Hydrolases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Structure of the manganese complex of photosystem II upon removal of the 33-kilodalton extrinsic pro...
Next Document: Inhibition of tubulin self-assembly and tubulin-colchicine GTPase activity by guanosine 5'-(gamma-fl...