Document Detail


Partial purification and characterization of the mRNA complementing a temperature-sensitive S-phase cell cycle mutation.
MedLine Citation:
PMID:  6201855     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
E36ts24 is a temperature-sensitive (ts) cell cycle mutant derived from the Chinese hamster lung cell line E36. At the restrictive temperature for growth (40.3 degrees C), the mutant cells are arrested at early S phase. We have microinjected poly(A)+ RNA isolated from the wild-type E36 cells into the cytoplasm of E36ts24 cells arrested at early S phase after 24 hr of incubation at 40.3 degrees C. The ts mutation was transiently complemented in a significant fraction of the microinjected cells as evidenced by the incorporation of [3H]thymidine assayed by autoradiography. Microinjection of mRNA fractionated by methylmercuric hydroxide/agarose gel showed that the mRNA capable of transiently complementing the mutation in E36ts24 contains about 940 nucleotides. Hence, it can code at most for a protein containing about 230 amino acids. We estimate that the partial purification by fractionation of the mRNA active in the transient complementation is on the order of 100- to 200-fold.
Authors:
A Fainsod; M Marcus; P F Lin; F H Ruddle
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  81     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1984 Apr 
Date Detail:
Created Date:  1984-06-07     Completed Date:  1984-06-07     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2393-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cricetinae
Cricetulus
Genetic Complementation Test
Interphase*
Lung
Molecular Weight
Mutation*
Poly A / genetics,  isolation & purification
RNA / genetics,  isolation & purification
RNA, Messenger / genetics*,  isolation & purification
Temperature
Thymidine Kinase / genetics
Chemical
Reg. No./Substance:
0/RNA, Messenger; 24937-83-5/Poly A; 63231-63-0/RNA; EC 2.7.1.21/Thymidine Kinase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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