Document Detail


Partial purification and characterization of human megakaryocyte colony-stimulating factor (Meg-CSF).
MedLine Citation:
PMID:  2324552     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Megakaryocyte colony-stimulating factor (Meg-CSF) in urinary extracts from patients with aplastic anemia was partially characterized and purified. Using Meg-CSF-enriched fractions, we established that the moiety has the following characteristics: 1) portions of the molecules having Meg-CSF activity have sialic acid, probably with a biantennary structure, and beta-galactose residues as the terminal and penultimate sugars; 2) disulfide residues are an essential chemical group of the molecule and are located on its surface; and 3) Meg-CSF activity is stable in n-propanol, but not in acetonitrile with trifluoroacetic acid. Partial purification of Meg-CSF by a four-step procedure of ethanol precipitation, CM Affi-Gel Blue chromatography, wheat germ agglutinin-sepharose chromatography, and high-resolution hydroxyapatite chromatography, yielded a concentrate with a 430- to 630-fold increase in specific activity. The partially purified Meg-CSF fractions stimulated both human and murine megakaryocytopoiesis in vitro (CFU-meg). When analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis under nonreduced conditions, Meg-CSF activity was recovered in the 29-34 kDa molecular weight fractions. We have also shown that Meg-CSF, purified from the urine of aplastic anemia patients, stimulated murine megakaryocytopoiesis and platelet production in vivo. Final purification of human urinary Meg-CSF is currently in progress.
Authors:
K Ogata; Z G Zhang; K Abe; M J Murphy
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  International journal of cell cloning     Volume:  8 Suppl 1     ISSN:  0737-1454     ISO Abbreviation:  Int. J. Cell Cloning     Publication Date:  1990 Jan 
Date Detail:
Created Date:  1990-05-21     Completed Date:  1990-05-21     Revised Date:  2011-10-26    
Medline Journal Info:
Nlm Unique ID:  8308172     Medline TA:  Int J Cell Cloning     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  103-20     Citation Subset:  IM    
Affiliation:
Hipple Cancer Research Center, Dayton, Ohio 45439-2092.
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MeSH Terms
Descriptor/Qualifier:
Anemia, Aplastic / urine
Animals
Blood Proteins / urine*
Chromatography, Affinity
Colony-Stimulating Factors / urine*
GPI-Linked Proteins
Humans
Megakaryocytes / analysis*
Membrane Glycoproteins
Neuraminidase
Proteins / isolation & purification*
Rats
Solvents
Sulfhydryl Reagents
Chemical
Reg. No./Substance:
0/Blood Proteins; 0/Colony-Stimulating Factors; 0/GPI-Linked Proteins; 0/Membrane Glycoproteins; 0/Proteins; 0/Solvents; 0/Sulfhydryl Reagents; 0/mesothelin; EC 3.2.1.18/Neuraminidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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