Document Detail


Partial purification and characterization of heat-stable enterotoxin produced by Yersinia enterocolitica.
MedLine Citation:
PMID:  7216460     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
By using a suckling mouse assay, heat-stable enterotoxin (ST) was purified from the culture filtrate of Yersinia enterocolitica isolated from a diarrheal patient. The purification procedures involve ultrafiltration with an Amicon HIP-10 hollow fiber, ethanol fractionation, protamine sulfate treatment, diethylaminoethyl-Sephacel and hydroxylapatite column chromatographies, and Sephacryl S-200 superfine gel filtration. About 408-fold purification was achieved, with a yield of 12.0%. The minimal effective dose of purified ST was about 110 ng in the suckling mouse assay. The molecular weight of purified ST was 9,000 by Sephadex G-100 superfine gel filtration. The purified ST was stable to heating (100 degrees C for 20 min, 121 degrees C for 20 min) and did not lose its toxicity after treatment with protease, trypsin, lipase, phospholipase C, ribonuclease, deoxyribonuclease, beta-glucosidase, and neuraminidase. The purified ST was separated by isoelectric focusing into two active fractions, with pI's of 3.29 (ST-1) and 3.00 (ST-2), respectively. Antiserum from guinea pigs immunized with the purified ST neutralized the activity of both Y. enterocolitica ST and Escherichia coli ST.
Authors:
K Okamoto; T Inoue; H Ichikawa; Y Kawamoto; A Miyama
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Infection and immunity     Volume:  31     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1981 Feb 
Date Detail:
Created Date:  1981-06-13     Completed Date:  1981-06-13     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  554-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacterial Toxins / immunology,  isolation & purification*
Biological Assay
Cross Reactions
Enterotoxins / immunology,  isolation & purification*
Hot Temperature
Isoelectric Point
Molecular Weight
Yersinia / analysis*,  immunology
Chemical
Reg. No./Substance:
0/Bacterial Toxins; 0/Enterotoxins
Comments/Corrections

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