| Partial purification and characterization of heat-stable enterotoxin produced by Yersinia enterocolitica. | |
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MedLine Citation:
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PMID: 7216460 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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By using a suckling mouse assay, heat-stable enterotoxin (ST) was purified from the culture filtrate of Yersinia enterocolitica isolated from a diarrheal patient. The purification procedures involve ultrafiltration with an Amicon HIP-10 hollow fiber, ethanol fractionation, protamine sulfate treatment, diethylaminoethyl-Sephacel and hydroxylapatite column chromatographies, and Sephacryl S-200 superfine gel filtration. About 408-fold purification was achieved, with a yield of 12.0%. The minimal effective dose of purified ST was about 110 ng in the suckling mouse assay. The molecular weight of purified ST was 9,000 by Sephadex G-100 superfine gel filtration. The purified ST was stable to heating (100 degrees C for 20 min, 121 degrees C for 20 min) and did not lose its toxicity after treatment with protease, trypsin, lipase, phospholipase C, ribonuclease, deoxyribonuclease, beta-glucosidase, and neuraminidase. The purified ST was separated by isoelectric focusing into two active fractions, with pI's of 3.29 (ST-1) and 3.00 (ST-2), respectively. Antiserum from guinea pigs immunized with the purified ST neutralized the activity of both Y. enterocolitica ST and Escherichia coli ST. |
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Authors:
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K Okamoto; T Inoue; H Ichikawa; Y Kawamoto; A Miyama |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Infection and immunity Volume: 31 ISSN: 0019-9567 ISO Abbreviation: Infect. Immun. Publication Date: 1981 Feb |
Date Detail:
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Created Date: 1981-06-13 Completed Date: 1981-06-13 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 554-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Toxins
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immunology,
isolation & purification* Biological Assay Cross Reactions Enterotoxins / immunology, isolation & purification* Hot Temperature Isoelectric Point Molecular Weight Yersinia / analysis*, immunology |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Toxins; 0/Enterotoxins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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