Document Detail


Partial purification and characterization of an escherichia coli toxic factor that induces morphological cell alterations.
MedLine Citation:
PMID:  6341235     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A factor produced by several strains of Escherichia coli isolated from enteritis-affected children has been shown to produce both a necrotizing effect on rabbit skin and striking morphological alterations on CHO, Vero, and HeLa cells. The same strains were found to have hemolytic activity on sheep erythrocytes. The toxic, cell-altering factor was demonstrated to be different from both heat-labile and heat-stable enterotoxins and from Vero toxin. The main effect induced by the isolated factor on cultured cells was the formation of large multinucleated cells. The partial purification achieved suggests that the same factor (most likely a protein with a molecular weight of 70,000 to 80,000) is responsible for toxic and cell-altering activities, whereas a different molecular species is responsible for hemolytic activity.
Authors:
A Caprioli; V Falbo; L G Roda; F M Ruggeri; C Zona
Related Documents :
16897295 - Assessing the contamination potential of freshly extracted escherichia coli biofilm cel...
21371235 - Neisseria gonorrhoeae pilin glycan contributes to cr3 activation during challenge of pr...
10085 - Identification of a ribonuclease p-like activity from human kb cells.
7007325 - Lipid synthesis during the escherichia coli cell cycle.
19084665 - Electrochemistry and chemiluminescence techniques compared in the detection of nadph ox...
18836915 - The effect of type i collagen on osteochondrogenic differentiation in adipose-derived s...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Infection and immunity     Volume:  39     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1983 Mar 
Date Detail:
Created Date:  1983-06-23     Completed Date:  1983-06-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1300-6     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Toxins / analysis,  isolation & purification*,  pharmacology
Cell Line
Cell Nucleus / ultrastructure
Cercopithecus aethiops
Cricetinae
Cytotoxins / analysis,  isolation & purification*,  pharmacology
Escherichia coli / analysis*
Female
Guinea Pigs
Hela Cells
Hemolysis
Hot Temperature
Humans
Hydrogen-Ion Concentration
Necrosis
Ovary
Rabbits
Sheep
Chemical
Reg. No./Substance:
0/Bacterial Toxins; 0/Cytotoxins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Relationship between cecal population levels of indigenous bacteria and translocation to the mesente...
Next Document:  Purification and characterization of a wall protein antigen from Clostridium botulinum type A.