Document Detail

Partial purification and characterization of an escherichia coli toxic factor that induces morphological cell alterations.
MedLine Citation:
PMID:  6341235     Owner:  NLM     Status:  MEDLINE    
A factor produced by several strains of Escherichia coli isolated from enteritis-affected children has been shown to produce both a necrotizing effect on rabbit skin and striking morphological alterations on CHO, Vero, and HeLa cells. The same strains were found to have hemolytic activity on sheep erythrocytes. The toxic, cell-altering factor was demonstrated to be different from both heat-labile and heat-stable enterotoxins and from Vero toxin. The main effect induced by the isolated factor on cultured cells was the formation of large multinucleated cells. The partial purification achieved suggests that the same factor (most likely a protein with a molecular weight of 70,000 to 80,000) is responsible for toxic and cell-altering activities, whereas a different molecular species is responsible for hemolytic activity.
A Caprioli; V Falbo; L G Roda; F M Ruggeri; C Zona
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Infection and immunity     Volume:  39     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1983 Mar 
Date Detail:
Created Date:  1983-06-23     Completed Date:  1983-06-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1300-6     Citation Subset:  IM    
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MeSH Terms
Bacterial Toxins / analysis,  isolation & purification*,  pharmacology
Cell Line
Cell Nucleus / ultrastructure
Cercopithecus aethiops
Cytotoxins / analysis,  isolation & purification*,  pharmacology
Escherichia coli / analysis*
Guinea Pigs
Hela Cells
Hot Temperature
Hydrogen-Ion Concentration
Reg. No./Substance:
0/Bacterial Toxins; 0/Cytotoxins

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