| Partial purification and characterization of an escherichia coli toxic factor that induces morphological cell alterations. | |
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MedLine Citation:
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PMID: 6341235 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A factor produced by several strains of Escherichia coli isolated from enteritis-affected children has been shown to produce both a necrotizing effect on rabbit skin and striking morphological alterations on CHO, Vero, and HeLa cells. The same strains were found to have hemolytic activity on sheep erythrocytes. The toxic, cell-altering factor was demonstrated to be different from both heat-labile and heat-stable enterotoxins and from Vero toxin. The main effect induced by the isolated factor on cultured cells was the formation of large multinucleated cells. The partial purification achieved suggests that the same factor (most likely a protein with a molecular weight of 70,000 to 80,000) is responsible for toxic and cell-altering activities, whereas a different molecular species is responsible for hemolytic activity. |
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Authors:
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A Caprioli; V Falbo; L G Roda; F M Ruggeri; C Zona |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Infection and immunity Volume: 39 ISSN: 0019-9567 ISO Abbreviation: Infect. Immun. Publication Date: 1983 Mar |
Date Detail:
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Created Date: 1983-06-23 Completed Date: 1983-06-23 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1300-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bacterial Toxins / analysis, isolation & purification*, pharmacology Cell Line Cell Nucleus / ultrastructure Cercopithecus aethiops Cricetinae Cytotoxins / analysis, isolation & purification*, pharmacology Escherichia coli / analysis* Female Guinea Pigs Hela Cells Hemolysis Hot Temperature Humans Hydrogen-Ion Concentration Necrosis Ovary Rabbits Sheep |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Toxins; 0/Cytotoxins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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